Structural basis for imipenem inhibition of class C β-lactamases

被引：64

作者：

Beadle, BM ^{[1
]}

Shoichet, BK ^{[1
]}

机构：

[1] Northwestern Univ, Dept Mol Pharmacol & Biol Chem, Chicago, IL 60611 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 12期

关键词：

D O I：

10.1128/AAC.46.12.3978-3980.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine how imipenem inhibits the class C beta-lactamase AmpC, the X-ray crystal structure of the acyl-enzyme complex was determined to a resolution of 1.80 Angstrom. In the complex, the lactam carbonyl oxygen of imipenem has flipped by approximately 180degrees compared to its expected position; the electrophilic acyl center is thus displaced from the point of hydrolytic attack. This conformation resembles that of imipenem bound to the class A enzyme TEM-1 but is different from that of moxalactam bound to AmpC.

引用

页码：3978 / 3980

页数：3

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