Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis

被引：21

作者：

Ohrman, Caroline ^{[1
]}

Sahl, Jason W. ^{[2
]}

Sjodin, Andreas ^{[1
]}

Uneklint, Ingrid ^{[1
]}

Ballard, Rebecca ^{[2
]}

Karlsson, Linda ^{[1
]}

McDonough, Ryelan F. ^{[2
]}

Sundell, David ^{[1
]}

Soria, Kathleen ^{[2
]}

Backman, Stina ^{[1
]}

Chase, Kitty ^{[3
]}

Brindefalk, Bjorn ^{[1
]}

Sozhamannan, Shanmuga ^{[4
]}

Vallesi, Adriana ^{[5
]}

Hagglund, Emil ^{[1
]}

Ramirez-Paredes, Jose Gustavo ^{[6
]}

Thelaus, Johanna ^{[1
]}

Colquhoun, Duncan ^{[7
]}

Myrtennas, Kerstin ^{[1
]}

Birdsell, Dawn ^{[2
]}

Johansson, Anders ^{[8
,9
]}

Wagner, David M. ^{[2
]}

Forsman, Mats ^{[1
]}

机构：

[1] Swedish Def Res Agcy, CBRN Def & Secur, FOI, SE-90182 Umea, Sweden

[2] No Arizona Univ, Pathogen & Microbiome Inst, Flagstaff, AZ 86011 USA

[3] US Army, Med Res Inst, Ft Detrick, MD 21702 USA

[4] CBRND Enabling Biotechnol JPL CBRND EB, Ft Detrick, MD 21702 USA

[5] Univ Camerino, Sch Biosci & Vet Med, I-62032 Camerino, Italy

[6] Ridgeway Biol Ltd, Units 1-3 Old Stn Business Pk, Compton RG20 6NE, Berks, England

[7] Norwegian Vet Inst, Fish Hlth Res Grp, Pb 750 Sentrum, N-0106 Oslo 23, Norway

[8] Umea Univ, Dept Clin Microbiol, Clin Bacteriol, SE-90185 Umea, Sweden

[9] Umea Univ, Lab Mol Infect Med Sweden MIMS, SE-90185 Umea, Sweden

来源：

MICROORGANISMS | 2021年 / 9卷 / 01期

关键词：

Francisella taxonomy; tularemia; phylogeny; assay;

D O I：

10.3390/microorganisms9010146

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of Francisella tularensis in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known Francisellaceae species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to F. tularensis that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of F. tularensis in environmental samples, such as water and air filters.

引用

页码：1 / 21

页数：21

共 3 条

[1] Development of real-time PCR assays for the specific detection of Francisella tularensis ssp tularensis, holarctica and mediaasiatica
Mitchell, Jan L.
Chatwell, Nicola
Christensen, Deanna
Diaper, Helen
Minogue, Timothy D.
Parsons, Tanya M.
Walker, Brian
Weller, Simon A.
MOLECULAR AND CELLULAR PROBES, 2010, 24 (02) : 72 - 76
[2] Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Buse, Helen Y.
Morris, Brian J.
Rice, Eugene W.
BMC MICROBIOLOGY, 2020, 20 (01)
[3] Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Helen Y. Buse
Brian J. Morris
Eugene W. Rice
BMC Microbiology, 20

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