New fluorogenic substrate for the first continuous steroid sulfatase assay

被引：15

作者：

Bilban, M ^{[1
]}

Billich, A ^{[1
]}

Auer, M ^{[1
]}

Nussbaumer, P ^{[1
]}

机构：

[1] NOVARTIS Res Inst, A-1235 Vienna, Austria

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 09期

关键词：

D O I：

10.1016/S0960-894X(00)00144-X

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The screening for new inhibitors of steroid sulfatase requires an efficient test system. To overcome the shortcomings of the available discontinuous fluorimetric assay, several coumarin-type compounds were investigated as potential new substrates. 3,4-Benzocoumarin 7-O-sulfate was found to have appropriate substrate properties for the establishment of the first direct continuous assay of steroid sulfatase. (C) 2000 Elsevier Science Ltd. All rights reserved.

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页码：967 / 969

页数：3

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