Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides

被引：7

作者：

Di Wei ^{[1
]}

Zhang Ying-Chun ^{[1
]}

Yi Hua-Xi ^{[2
]}

Han Xue ^{[1
]}

Wang Shu-Mei ^{[3
]}

Zhang Lan-Wei ^{[1
,2
]}

机构：

[1] Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150090, Heilongjiang, Peoples R China

[2] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

[3] Harbin Univ, Sch Food Sci & Engn, Harbin 150086, Heilongjiang, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2018年 / 46卷 / 06期

关键词：

Lactic acid bacteria; Exopolysaccharides; Isolation and purification; Structure analysis; Review; LACTOBACILLUS-CASEI LC2W; ANTITUMOR-ACTIVITY; PHYSICOCHEMICAL CHARACTERIZATION; ANTIOXIDANT ACTIVITIES; NMR-SPECTROSCOPY; KEFIR GRAINS; POLYSACCHARIDES; MILK; BIOACTIVITIES; PURIFICATION;

D O I：

10.1016/S1872-2040(18)61091-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Exopolysaccharides (EPSs), produced by lactic acid bacteria (LAB), have been used primarily to improve the quality and taste of food, also possess a variety of unique biological functions, such as immunoregulation and anti-tumor activities. The diversity of molecular structural characteristics of LAB-generated EPSs represents one of the main factors responsible for this plethora of functions. Accordingly, the structural analysis of the EPSs produced by LAB is the prerequisite and basis for examining its functional and structure-activity relationships. In this report, we summarized the current progress of key methodologies involved in the structural analysis of LAB-generated EPSs, including their isolation, purification, primary structure and advances in structural research. A comprehensive discussion regarding the application of chemical analysis, instrument analysis and computer aided technology in the structure analysis of LAB-generated EPSs was provided. Further, the future development of the research on the structure of LAB-generated EPSs was also presented.

引用

页码：875 / 882

页数：7