Suppression of AGE precursor formation following unilateral ureteral obstruction in mouse kidneys by transgenic expression of α-dicarbonyl/L-xylulose reductase

被引：11
作者：
Asami, Jun [1 ]
Odani, Hiroko [1 ]
Ishii, Aiko [1 ]
Oide, Kayoko [1 ]
Sudo, Takako [1 ]
Nakamura, Atsushi [1 ]
Miyata, Noriyuki [1 ]
Otsuka, Noboru [1 ]
Maeda, Kenji [1 ]
Nakagawa, Junichi [1 ]
机构：
[1] Nagoya Univ, Sch Med, Dept In Home Med, Nagoya, Aichi 4668560, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 12期
关键词：
advanced glycation endproduct; unilateral ureteral obstruction; transgenic mice; 3-deoxyglucosone; dicarbonyl reductase;
D O I：
10.1271/bbb.60311
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Unilateral ureteral obstruction (UUO) of kidneys causes acute generation of carbonyl stress. By electrospray ionization/liquid chromatography/mass spectrometry (ESI/LC/MS) we measured the content of methyl glyoxal, glyoxal, and 3-deoxyglucosone in mouse kidney extracts following UUO. UUO resulted in elevation of these dicarbonyls in the obstructed kidneys. Furthermore, the accumulation of 3-deoxyglucosone was significantly reduced in the kidneys of mice transgenic for alpha-dicarbonyl/L-xylulose reductase (DCXR) as compared to their wild-type littermates, demonstrating 4.91 +/- 2.04 vs. 6.45 +/- 1.85 ng/mg protein (P = 0.044) for the obstructed kidneys, and 3.68 +/- 1.95 vs. 5.20 +/- 1.39 ng/mg protein (P = 0.026) for the contralateral kidneys. On the other hand, collagen III content in kidneys showed no difference as monitored by in situ hybridization. Collectively, DCXR may function in the removal of renal et-dicarbonyl compounds under oxidative circumstances, but it was not sufficient to suppress acute renal fibrosis during 7 d of UUO by itself.
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页码：2899 / 2905
页数：7
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