Micellar electrokinetic capillary chromatography of benzodiazepine antiepileptics and their desmethyl metabolites in blood

被引：22
作者：
Imazawa, M
Hatanaka, Y
机构：
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 15卷 / 9-10期
关键词：
micellar electrokinetic chromatography; benzodiazepines; antiepileptics; human serum; electrophoresis; sample stacking; sodium dodecylsulphate; serum;
D O I：
10.1016/S0731-7085(97)00037-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A fast and reliable method for the MEKC separation and determination of benzodiazepine antipileptics and their active metabolites in serum has been developed, using a separation buffer composed of berate (pH 9.5), SDS (18 mM), and acetonitrile (14%) as an organic modifier. The method is sensitive enough to be used clinically with a precision of less than 3% for the analysis of nitrazepam. clonazepam, clobazam, diazepam and their desmethyl metabolites in serum. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：1503 / 1508
页数：6
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