Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte

被引：4

作者：

Kong, Qing-ran ^{[1
]}

Zhang, Jia-ming ^{[1
]}

Zhang, Xiao-lei ^{[1
]}

Zong, Ming ^{[1
]}

Zheng, Kai-lun ^{[1
]}

Liu, Lei ^{[1
]}

Meng, Fan-xi ^{[1
]}

Zhang, Ting-ting ^{[1
]}

Weng, Xiao-gang ^{[1
]}

Mu, Yan-shuang ^{[1
]}

Liu, Zhong-hua ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Life Sci, Key Lab Anim Cellular & Genet Engn Heilongjiang P, Harbin 150030, Heilongjiang, Peoples R China

来源：

THERIOGENOLOGY | 2019年 / 135卷

关键词：

Pig; Oocyte; SINE1B; endo-siRNAs; IVM; SMALL INTERFERING RNAS; MOUSE; RETROTRANSPOSONS; IDENTIFICATION; TRANSCRIPTS; SENSE;

D O I：

10.1016/j.theriogenology.2019.05.011

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Approximately 40% of mammalian genome is made of transposable elements (TEs), and during specific biological processes, such as gametogenesis, they may be activated by global demethylation, so strict silencing mechanism is indispensable for genomic stability. Here, we performed small RNA-seq on Dicer1 knockdown (KD) oocytes in pig, and observed short interspersed nuclear elements 18 (SINE1B) derived endogenous small interfering RNAs (endo-siRNAs), termed SINE1B-siRNAs, were significantly decreased and their biogenesis was dependent on Dicer1 and transcript of SINE1B. Furthermore, by injection of mimics and inhibitors of the SINE1B-siRNAs into germinal vesicle-stage (GV-stage) oocytes, we found the maturation rate was significantly decreased by SINE1B-siRNAs, indicating the SINE1B-siRNAs are indispensible for in vitro maturation (IVM) of porcine oocyte. To figure out the mechanism, we checked the expression pattern and DNA methylation status of SINE1B during IVM of porcine oocytes, and demonstrated the SINE1B-siRNAs could repress SINE1B expression induced by hypomethylation at a post transcriptional level. Our results suggest that during gametogenesis when the erasure of DNA methylation occurs, endo-siRNAs act as a chronic response to limit retrotransposon activation. (C) 2019 Elsevier Inc. All rights reserved.

引用

页码：19 / 24

页数：6

共 31 条

[1] An endosiRNA-Based Repression Mechanism Counteracts Transposon Activation during Global DNA Demethylation in Embryonic Stem Cells [J].