Highly efficient in vitro regeneration methodology for mature Chinese tallow tree (Sapium sebiferum Roxb)

A highly efficient in vitro regeneration methodology for mature chinese tallow tree (Sapium sebiferum Roxb.) has been developed. Shoot segments cultured on MS medium supplemented with 7.5 mu M NAA produced light green callus. Optimum shoot differentiation resulted when callus was transferred to MS medium with 1 mu M BA and 0.25 mu M NAA. Shoot forming ability of callus was higher on MS medium compared to B5, half-MS or WPM. A continuous shoot harvest system at four-week intervals was established. Shoot yield continued for six months without loss of vigour. Regenerated shoots were rooted by culturing on half-strength agar-gelled MS medium containing 1 mu M IBA. Rooted plantlets were transferred to 1:1 soil vermiculite mixture and acclimatized with 67 % survival rate. Fully acclimatized plants were planted in the field, and performance is being evaluated.