Pseudomonas lalkuanensis sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste

A novel e-waste-degrading strain, PE08(T), was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08(T) was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 degrees C (range, 5-40 degrees C), with 1-2% NaCl (range, 0-3 %) and at pH 7 (range 6-11). The phylogeny based on 16S rRNA gene sequences delineated strain PE08(T) to the genus Pseudomonas and showed highest sequence similarity to Pseudomonas furukawaii KF707(T) (98.70 %), followed by Pseudomonas aeruginosa DSM 50071(T) (98.62 %) and Pseudomonas resinovorans DSM 21078(T) (97.93 %). The genome of strain PE08(T) was sequenced and had one scaffold of 6056953 bp, 99.84 % completeness and 182x coverage were obtained. The G+C content in the genome was 64.24mol%. The DNA-DNA hybridization and average nucleotide identity values between strain PE08(T) and its closely related type strain, P. resinovorans DSM 21078(T) were below 34.8 % and 87.96 %, respectively. The phylogenetic analysis based on whole-genome sequence and concatenated GyrB and RpoB proteins revealed that strain PE08(T) forms a district clade in the family Pseudomonadaceae. The predominant fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and C-12:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The phenotypic, chemotaxonomic and genetic analysis, including overall genome relatedness index values, indicated that strain PE08(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas lalkuanensis sp. nov. is proposed. The type strain is PE08(T) (=MCC 3792=KCTC 72454=CCUG 73691).