Photo-immobilization of bone morphogenic protein 2 on PLGA/HA nanocomposites to enhance the osteogenesis of adipose-derived stem cells

被引：28

作者：

Gao, Tianlin ^{[1
,2
]}

Cui, Weiwei ^{[2
]}

Wang, Zongliang ^{[1
]}

Wang, Yu ^{[1
]}

Liu, Ya ^{[2
]}

Malliappan, Ponnurengam Sivakumar ^{[3
,4
]}

Ito, Yoshihiro ^{[3
,4
]}

Zhang, Peibiao ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China

[2] Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China

[3] RIKEN, Nano Med Engn Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[4] RIKEN, Ctr Emergent Mat Sci, Emergent Bioengn Mat Res Team, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

来源：

RSC ADVANCES | 2016年 / 6卷 / 24期

基金：

中国国家自然科学基金;

关键词：

NANO-HYDROXYAPATITE; STROMAL CELLS; DIFFERENTIATION; REGENERATION; GELATIN; GROWTH; PROLIFERATION; NANOFIBERS; COMPOSITE; SCAFFOLD;

D O I：

10.1039/c5ra27914c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lack of bioactivity has seriously restricted the development of biodegradable implants in bone tissue engineering. In this study, Bone Morphogenic Protein 2 (BMP-2) was photo-immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA composites via photo-reactive gelatin (Az-Gel). The results demonstrate that the Az-Gel facilitates efficient and long-term immobilization of peptides on the surface of PLGA. The immobilization of Az-Gel enhanced the adhesion and proliferation of Mouse Adipose-Derived Stem Cells (ADSCs), while HA incorporation facilitated cellular osteodifferentiation. The immobilization of BMP-2 induced the osteogenesis of the ADSCs, indicated by alkaline phosphatase activity, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and mineralization on the deposited substrates. Furthermore, the surface immobilization of BMP-2 could not only reduce the amount of growth factor, but also have a long-term osteoinductive effect. Therefore, it is considered that the surface modification method has a great potential for the enhancement of osteointegration of biodegradable bone implants.

引用

页码：20202 / 20210

页数：9

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