Quantitative mRNA expression of sox3 and DMRT1 during sex reversal, and expression profiles after GnRHa administration in black porgy, Acanthopagrus schlegeli

被引：31
作者：
Shin, Hyun Suk [1 ]
An, Kwang Wook [1 ]
Park, Mi Seon [2 ]
Jeong, Min Hwan [3 ]
Choi, Cheol Young [1 ]
机构：
[1] Korea Maritime Univ, Div Marine Environm Biosci, Pusan 606791, South Korea
[2] Natl Fisheries & Dev Inst, Res & Dev Planning Dept, Pusan 619705, South Korea
[3] Natl Fisheries Res & Dev Inst, Aquaculture Management Div, Pusan 619902, South Korea
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2009年 / 154卷 / 01期
关键词：
Black porgy; DMRT1; sox3; Sex reverse; GnRHa; MOLECULAR-CLONING; GENES; BASS; SPERMATOGENESIS; DIFFERENTIATION; TRANSCRIPTION; CONSERVATION; CHROMOSOME; EVOLUTION; AROMATASE;
D O I：
10.1016/j.cbpb.2009.05.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We cloned full-length sox3 cDNA from testis of black porgy, Acanthopagrus schlegeli. Black porgy sox3 cDNA consists of 897 base pairs (bp) and encodes a protein of 298 amino acids. We have investigated the expression pattern of sox3 and DMRT1 mRNA during the sex-reverse process from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary and ovary). The expression of sox3 and DMRT1 mRNA was high in mature testis of black porgy during sex-reverse process. In a histological analysis, testicular portion of gonad was degenerated and the ovary portion was increased during sex reversal from male to female, and then oocytes were increased in ovary. Also we examined the expression of sox3 and DMRT1 mRNA after gonadotropin-releasing hormone analogue (GnRHa) treatment in immature black porgy. The expression of sox3 and DMRT1 mRNA was increased after GnRHa treatment (in vivo and in vitro experiment) in immature black porgy. Therefore, we concluded that sox3 and DMRT1 were involved in the development of testis than ovary in black porgy. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：150 / 156
页数：7
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