Cotton cultivars Qalandri and MNH-93 were analysed for their heat acclimation potential. The 812 days old leaves were given repeated hardening treatments at 42degreesC for 2h for upto 7 days and after every treatment the leaves were heat stressed at 44degreesC for 2h. The heat acclimation potential was analysed after 24h recovery period at 30degreesC. The results showed that cv. MNH-93 has fairly good acclimation potential than cv. Qalandri by recovering 63% Fv/Fm ratio as compared 50% in cv. Qalandri indicating that adaptive changes in PS-II were more effective in cv. MNH-93 than cv. Qalandri.