Isolation and Characterization of Gardnerella Phage vB_Gva_AB1, a Bacteriophage Infecting a Clinical Strain of Gardnerella vaginalis

被引:3
作者
Bordigoni, Alexia [1 ]
Bouchard, Sonia [2 ]
Desnues, Christelle [1 ,2 ]
机构
[1] Aix Marseille Univ, AP HM, IRD 198, UMR Microbes Evolut Phylogeny & Infect MEPHI,IHU, Marseille, France
[2] Univ Toulon & Var, Aix Marseille Univ, Mediterranean Inst Oceanog, CNRS,IRD,UM 110, Marseille, France
关键词
BACTERIAL VAGINOSIS; DOUBLE-BLIND; PROBIOTICS; VAGINITIS; THERAPY;
D O I
10.1128/MRA.00053-21
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Gardnerella vaginalis is the presumed causative agent of bacterial vaginosis. Here, we describe the complete genome sequence of Gardnerella phage vB_Gva_AB1, induced from a vaginal bacterial strain from a woman suffering with bacterial vaginosis. The phage double-stranded DNA (dsDNA) genome is 50,268 bp long with a GC content of 39.55% and contains 62 predicted open reading frames.
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页数:5
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共 17 条
[1]   GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions [J].
Besemer, J ;
Lomsadze, A ;
Borodovsky, M .
NUCLEIC ACIDS RESEARCH, 2001, 29 (12) :2607-2618
[2]  
Borodovsky Mark, 2011, Curr Protoc Bioinformatics, VChapter 4, DOI 10.1002/0471250953.bi0405s35
[3]   Efficacy of Oral Metronidazole with Vaginal Clindamycin or Vaginal Probiotic for Bacterial Vaginosis: Randomised Placebo-Controlled Double-Blind Trial [J].
Bradshaw, Catriona S. ;
Pirotta, Marie ;
De Guingand, Deborah ;
Hocking, Jane S. ;
Morton, Anna N. ;
Garland, Suzanne M. ;
Fehler, Glenda ;
Morrow, Andrea ;
Walker, Sandra ;
Vodstrcil, Lenka A. ;
Fairley, Christopher K. .
PLOS ONE, 2012, 7 (04)
[4]   High recurrence rates of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence [J].
Bradshaw, CS ;
Morton, AN ;
Hocking, J ;
Garland, SM ;
Morris, MB ;
Moss, LM ;
Horvath, LB ;
Kuzevska, I ;
Fairley, CK .
JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (11) :1478-1486
[5]  
Chan PP, 2019, METHODS MOL BIOL, V1962, P1, DOI 10.1007/978-1-4939-9173-0_1
[6]   CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins [J].
Couvin, David ;
Bernheim, Aude ;
Toffano-Nioche, Claire ;
Touchon, Marie ;
Michalik, Juraj ;
Neron, Bertrand ;
Rocha, Eduardo P. C. ;
Vergnaud, Gilles ;
Gautheret, Daniel ;
Pourcel, Christine .
NUCLEIC ACIDS RESEARCH, 2018, 46 (W1) :W246-W251
[7]   HAEMOPHILUS VAGINALIS VAGINITIS - A NEWLY DEFINED SPECIFIC INFECTION PREVIOUSLY CLASSIFIED NONSPECIFIC VAGINITIS [J].
GARDNER, HL ;
DUKES, CD .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1955, 69 (05) :962-976
[8]   Supplementation of standard antibiotic therapy with oral probiotics for bacterial vaginosis and aerobic vaginitis: a randomised, double-blind, placebo-controlled trial [J].
Heczko, Piotr B. ;
Tomusiak, Anna ;
Adamski, Pawel ;
Jakimiuk, Artur J. ;
Stefanski, Grzegorz ;
Mikolajczyk-Cichonska, Aleksandra ;
Suda-Szczurek, Magdalena ;
Strus, Magdalena .
BMC WOMENS HEALTH, 2015, 15
[9]   The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic [J].
Hill, Colin ;
Guarner, Francisco ;
Reid, Gregor ;
Gibson, Glenn R. ;
Merenstein, Daniel J. ;
Pot, Bruno ;
Morelli, Lorenzo ;
Canani, Roberto Berni ;
Flint, Harry J. ;
Salminen, Seppo ;
Calder, Philip C. ;
Sanders, Mary Ellen .
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY, 2014, 11 (08) :506-514
[10]   The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota [J].
Lagier, Jean-Christophe ;
Hugon, Perrine ;
Khelaifia, Saber ;
Fournier, Pierre-Edouard ;
La Scola, Bernard ;
Raoult, Didier .
CLINICAL MICROBIOLOGY REVIEWS, 2015, 28 (01) :237-264