Hard x-ray phase contrast imaging of black lipid membranes

被引：10
作者：
Beerlink, A. [1 ]
Mell, M. [1 ]
Tolkiehn, M. [1 ]
Salditt, T. [1 ]
机构：
[1] Univ Gottingen, Inst Rontgenphys, D-37077 Gottingen, Germany
来源：
APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 20期
关键词：
domains; lipid bilayers; molecular biophysics; organic compounds; X-ray diffraction; X-ray imaging; SYNCHROTRON-RADIATION; REFRACTIVE LENSES; MICROSCOPY; MICROTOMOGRAPHY;
D O I：
10.1063/1.3263946
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We report hard x-ray phase contrast imaging of black lipid membranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized.
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页数：3
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共 18 条
  • [1] [Anonymous], 2001, ELEMENTS MODERN XRAY
  • [2] X-ray structure analysis of free-standing lipid membranes facilitated by micromachined apertures
    Beerlink, Andre
    Wilbrandt, P. -J.
    Ziegler, E.
    Carbone, D.
    Metzger, T. H.
    Salditt, Tim
    [J]. LANGMUIR, 2008, 24 (09) : 4952 - 4958
  • [3] Phase objects in synchrotron radiation hard x-ray imaging
    Cloetens, P
    Barrett, R
    Baruchel, J
    Guigay, JP
    Schlenker, M
    [J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1996, 29 (01) : 133 - 146
  • [4] Hard x-ray phase imaging using simple propagation of a coherent synchrotron radiation beam
    Cloetens, P
    Ludwig, W
    Baruchel, J
    Guigay, JP
    Pernot-Rejmánková, P
    Salomé-Pateyron, M
    Schlenker, M
    Buffière, JY
    Maire, E
    Peix, G
    [J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1999, 32 (10A) : A145 - A151
  • [5] PHASE-CONTRAST IMAGING OF WEAKLY ABSORBING MATERIALS USING HARD X-RAYS
    DAVIS, TJ
    GAO, D
    GUREYEV, TE
    STEVENSON, AW
    WILKINS, SW
    [J]. NATURE, 1995, 373 (6515) : 595 - 598
  • [6] Phase contrast hard x-ray microscopy with submicron resolution
    Lagomarsino, S
    Cedola, A
    Cloetens, P
    DiFonzo, S
    Jark, W
    Soullie, G
    Riekel, C
    [J]. APPLIED PHYSICS LETTERS, 1997, 71 (18) : 2557 - 2559
  • [7] A microscope for hard x rays based on parabolic compound refractive lenses
    Lengeler, B
    Schroer, CG
    Richwin, M
    Tümmler, J
    Drakopoulos, M
    Snigirev, A
    Snigireva, I
    [J]. APPLIED PHYSICS LETTERS, 1999, 74 (26) : 3924 - 3926
  • [8] X-ray phase-contrast microscopy and microtomography
    Mayo, SC
    Davis, TJ
    Gureyev, TE
    Miller, PR
    Paganin, D
    Pogany, A
    Stevenson, AW
    Wilkins, SW
    [J]. OPTICS EXPRESS, 2003, 11 (19): : 2289 - 2302
  • [9] Characterization of biological thin films at the solid-liquid interface by X-ray reflectivity
    Miller, CE
    Majewski, J
    Gog, T
    Kuhl, TL
    [J]. PHYSICAL REVIEW LETTERS, 2005, 94 (23)
  • [10] RECONSTITUTION OF CELL MEMBRANE STRUCTURE IN VITRO AND ITS TRANSFORMATION INTO AN EXCITABLE SYSTEM
    MUELLER, P
    RUDIN, DO
    TIEN, HT
    WESCOTT, WC
    [J]. NATURE, 1962, 194 (4832) : 979 - &
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