Antisense down regulation of NtBI-1 in tobacco BY-2 cells induces accelerated cell death upon carbon starvation

被引：40

作者：

Bolduc, N ^{[1
]}

Brisson, LF ^{[1
]}

机构：

[1] Univ Laval, Dept Biochem & Microbiol, Quebec City, PQ G1K 7P4, Canada

来源：

FEBS LETTERS | 2002年 / 532卷 / 1-2期

关键词：

Bax inhibitor-1; programmed cell death; DNA fragmentation; plant; autophagy;

D O I：

10.1016/S0014-5793(02)03650-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bax inhibitor-1 (BI-1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti-apoptotic function of plant BI-1 by antisense (AS) down regulation of NtBI-1 in Nicotiana tabacum cv. BY-2 cells. We observed that AS cell lines were more susceptible to autophagy, internucleosomal DNA fragmentation and death than control cells when subjected to sucrose starvation and hypo-osmotic shock, in agreement with a role of BI-1 as a death inhibitor. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：111 / 114

页数：4

共 14 条

[1]

BOLDUC M, 2002, IN PRESS PLANTA

[2] Ethylene- and enzyme-mediated superoxide production and cell death in carrot cells grown under carbon starvation [J].

Chae, HS ;

Lee, WS .

PLANT CELL REPORTS, 2001, 20 (03) :256-261

[3] Plant programmed cell death: A common way to die [J].

Danon, A ;

Delorme, V ;

Mailhac, N ;

Gallois, P .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2000, 38 (09) :647-655

[4] Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response [J].

Delledonne, M ;

Zeier, J ;

Marocco, A ;

Lamb, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (23) :13454-13459

[5] Apoptosis: Live or die - Hard work either way! [J].

Gallaher, BW ;

Hille, R ;

Raile, K ;

Kiess, W .

HORMONE AND METABOLIC RESEARCH, 2001, 33 (09) :511-519

[6] Differential expression of putative cell death regulator genes in near-isogenic, resistant and susceptible barley lines during interaction with the powdery mildew fungus [J].

Hückelhoven, R ;

Dechert, C ;

Trujillo, M ;

Kogel, KH .

PLANT MOLECULAR BIOLOGY, 2001, 47 (06) :739-748

[7] Evolutionally conserved plant homologue of the Bax Inhibitor-1 (BI-1) gene capable of suppressing Bax-induced cell death in yeast [J].

Kawai, M ;

Pan, L ;

Reed, JC ;

Uchimiya, H .

FEBS LETTERS, 1999, 464 (03) :143-147

[8] Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBl-1) [J].

Kawai-Yamada, M ;

Jin, LH ;

Yoshinaga, K ;

Hirata, A ;

Uchimiya, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (21) :12295-12300

[9] Autophagy in tobacco suspension-cultured cells in response to sucrose starvation [J].

Moriyasu, Y ;

Ohsumi, Y .

PLANT PHYSIOLOGY, 1996, 111 (04) :1233-1241

[10] SIMPLIFICATION OF PROTEIN ASSAY METHOD OF LOWRY ET AL - WHICH IS MORE GENERALLY APPLICABLE [J].

PETERSON, GL .

ANALYTICAL BIOCHEMISTRY, 1977, 83 (02) :346-356

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