A new member of the AKH/RPCH family that stimulates locomotory activity in the firebug, Pyrrhocoris apterus (Heteroptera)

被引：100
作者：
Kodrík, D
Socha, R
Simek, P
Zemek, R
Goldsworthy, GJ
机构：
[1] Acad Sci Czech Republic, Inst Entomol, CZ-37005 Ceske Budejovice, Czech Republic
[2] Univ London Birkbeck Coll, Dept Biol, London WC1E 7HX, England
来源：
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 30卷 / 06期
关键词：
adipokinetic hormone; AKH/RPCH; neuropeptide; HPLC; electrospray mass spectrometry; lipid mobilisation; locomotory activity; bug;
D O I：
10.1016/S0965-1748(00)00025-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new member of the AKH/RPCH family was isolated and identified from the corpora cardiaca of the firebug Pyrrhocoris apterus. The peptide was isolated in a single step by reversed phase HPLC and the structure deduced from the multiple MS (MS(N)) electrospray mass spectra and amino acid analysis as that of an octapeptide with the sequence pGlu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-NH(2): this sequence was confirmed by synthesis. The synthetic peptide induced lipid mobilisation and stimulated locomotory activity in macropterous females. This peptide, designated as Pyrrhocoris apterus adipokinetic hormone (Pya-AKH), is the first identified adipokinetic hormone described in a representative species of the suborder Heteroptera. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：489 / 498
页数：10
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