Spurious Amplification of a Plasmodium vivax Small-Subunit RNA Gene by Use of Primers Currently Used To Detect P. knowlesi

被引：144

作者：

Imwong, Mallika ^{[1
]}

Tanomsing, Naowarat ^{[1
]}

Pukrittayakamee, Sasithon ^{[1
,2
]}

Day, Nicholas P. J. ^{[1
,3
]}

White, Nicholas J. ^{[1
,3
]}

Snounou, Georges ^{[4
,5
,6
]}

机构：

[1] Mahidol Univ, Fac Trop Med, Dept Clin Trop Med, Bangkok, Thailand

[2] Royal Inst, Bangkok, Thailand

[3] Churchill Hosp, Ctr Trop Med, Oxford OX3 7LJ, England

[4] Hop La Pitie Salpetriere, INSERM, UMR S 945, Dept Parasitol, Paris, France

[5] Univ Paris 06, Paris, France

[6] Natl Univ Singapore, Fac Med, Dept Microbiol, Lab Mol & Cellular Parasitol, Singapore 117548, Singapore

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 12期

基金：

英国惠康基金;

关键词：

MALARIA; HUMANS; INFECTIONS; MALAYSIA; PARASITES; TRAVELER; THAILAND;

D O I：

10.1128/JCM.00811-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

引用

页码：4173 / 4175

页数：3

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