In situ imaging of the bacterial flagellar motor disassembly and assembly processes

被引:36
作者
Kaplan, Mohammed [1 ]
Subramanian, Poorna [1 ]
Ghosal, Debnath [1 ]
Oikonomou, Catherine M. [1 ]
Pirbadian, Sahand [2 ]
Starwalt-Lee, Ruth [3 ]
Mageswaran, Shrawan Kumar [1 ]
Ortega, Davi R. [1 ]
Gralnick, Jeffrey A. [3 ,4 ]
El-Naggar, Mohamed Y. [2 ]
Jensen, Grant J. [1 ,5 ]
机构
[1] CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA
[2] Univ Southern Calif, Dept Phys & Astron, Biol Sci & Chem, Los Angeles, CA USA
[3] Univ Minnesota Twin Cities, BioTechnol Inst, St Paul, MN USA
[4] Univ Minnesota Twin Cities, Dept Plant & Microbial Biol, St Paul, MN USA
[5] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA
基金
美国国家卫生研究院;
关键词
assembly; bacterial flagellar motor; disassembly; electron cryo-tomography; insitu imaging; CYCLE-DEPENDENT DEGRADATION; HOOK-BASAL-BODY; MOLECULAR ARCHITECTURE; SALMONELLA-TYPHIMURIUM; PROTEIN; CELL; IDENTIFICATION; TOMOGRAPHY; MEMBRANE; PEPTIDE;
D O I
10.15252/embj.2018100957
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The self-assembly of cellular macromolecular machines such as the bacterial flagellar motor requires the spatio-temporal synchronization of gene expression with proper protein localization and association of dozens of protein components. In Salmonella and Escherichia coli, a sequential, outward assembly mechanism has been proposed for the flagellar motor starting from the inner membrane, with the addition of each new component stabilizing the previous one. However, very little is known about flagellar disassembly. Here, using electron cryo-tomography and sub-tomogram averaging of intact Legionella pneumophila, Pseudomonas aeruginosa, and Shewanella oneidensis cells, we study flagellar motor disassembly and assembly insitu. We first show that motor disassembly results in stable outer membrane-embedded sub-complexes. These sub-complexes consist of the periplasmic embellished P- and L-rings, and bend the membrane inward while it remains apparently sealed. Additionally, we also observe various intermediates of the assembly process including an inner-membrane sub-complex consisting of the C-ring, MS-ring, and export apparatus. Finally, we show that the L-ring is responsible for reshaping the outer membrane, a crucial step in the flagellar assembly process.
引用
收藏
页数:12
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