Phenylethanoid glycosides from Paraboea martinii protect rat pheochromocytoma (PC12) cells from hydrogen peroxide-induced cell injury

被引：11

作者：

Gong, Xue ^{[1
]}

Xu, Yan ^{[2
]}

Ren, Kai ^{[3
]}

Bai, Xiaorong ^{[4
]}

Zhang, Chunhong ^{[1
]}

Li, Minhui ^{[1
,3
,5
,6
]}

机构：

[1] Baotou Med Coll, Dept Pharm, Baotou, Peoples R China

[2] Inner Mongolia Univ Sci & Technol, Affiliated Hosp 1, Baotou Med Coll, Baotou, Peoples R China

[3] Inner Mongolia Autonomous Reg Hosp Tradit Chinese, Intens Care Unit, Hohhot, Peoples R China

[4] Xilinggol Vocat Coll, Dept Med, Inner Mongolia, Peoples R China

[5] Guangxi Bot Garden Med Plants, Guangxi Key Lab Med Resources Protect & Genet Imp, Nanning, Peoples R China

[6] Inner Mongolia Inst Tradit Chinese Med, Pharmaceut Lab, Hohhot, Peoples R China

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2019年 / 83卷 / 12期

关键词：

Paraboea martinii; phenylethanoid glycosides; neuroprotective effects; zinc protoporphyrin; HO-1; OXIDATIVE STRESS; IDENTIFICATION; EXPRESSION; APOPTOSIS; PATHWAY; CANCER;

D O I：

10.1080/09168451.2019.1654359

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we isolated eight phenylethanoid glycosides from Paraboea martinii for the first time, and evaluated the mechanism underlying their neuroprotective effects against H2O2-induced injury in PC12 cells. The MTS method was utilized to screen the phenylethanoid glycosides for protective ability. Next, qRT-PCR and western blotting analysis were used to detect the transcription levels of HO-1 and GCLC, which are regulated by Nrf2. The inhibitor ZnPP was used to analyze the involvement of Nrf2 in HO-1 expression. Analyses showed that caleolarioside B, paraboside B, and paraboside II also upregulated the expression of HO-1, but showed no obvious effect on GCLC. Pretreatment with ZnPP significantly reduced the neuroprotective effects. Thus, phenylethanoid glycosides isolated from P. martinii protected PC12 cells from H2O2-induced damage by upregulating HO-1. The results provided evidence that P. martinii might be a potential therapeutic agent for the treatment of neurodegenerative diseases.

引用

页码：2202 / 2212

页数：11

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