Xenopus Axin-Related Protein: A Link Between Its Centrosomal Localization and Function in the Wnt/β-Catenin Pathway

被引：13

作者：

Alexandrova, Evguenia M. ^{[1
]}

Sokol, Sergei Y. ^{[1
]}

机构：

[1] Mt Sinai Sch Med, Dept Dev & Regenerat Biol, New York, NY 10029 USA

来源：

DEVELOPMENTAL DYNAMICS | 2010年 / 239卷 / 01期

基金：

美国国家卫生研究院;

关键词：

XARP; centrosome; Xenopus; DIX; Dsh; Axin; Wnt; WNT SIGNALING PATHWAY; BETA-CATENIN; DISEASE; PHOSPHORYLATION; EMBRYOGENESIS; SPECIFICITY; CILIARY; COMPLEX; CANCER; CELLS;

D O I：

10.1002/dvdy.22125

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The Wnt/beta-catenin signaling pathway regulates cell proliferation and cell fate determination in multiple systems. However, the subcellular localization of Wnt pathway components and the significance of this localization for the pathway regulation have not been extensively analyzed. Here we report that Xenopus Axin-related protein (XARP), a component of the beta-catenin destruction complex, is localized to the centrosome. This localization of XARP requires the presence of the DIX domain and an adjacent region. Since other components of the Wnt pathway have also been shown to associate with the centrosome, we tested a hypothesis that the beta-catenin destruction complex operates at the centrosome. However, XARP mutants with poor centrosomal localization revealed an enhanced rather than decreased ability to antagonize the Wnt/beta-catenin pathway. Our data are consistent with the idea that the inactivation of XARP at the centrosome is an important regulatory point in Wnt signaling. Developmental Dynamics 239:261-270, 2010. (C) 2009 Wiley-Liss, Inc.

引用

页码：261 / 270

页数：10

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