DNA Helicase Activity in Purified Human RECQL4 Protein

被引：40

作者：

Suzuki, Takahiro ^{[1
]}

Kohno, Toshiyuki ^{[2
]}

Ishimi, Yukio ^{[1
,2
]}

机构：

[1] Ibaraki Univ, Mito, Ibaraki 3108512, Japan

[2] Mitsubishi Kagaku Inst Life Sci MITILS, Tokyo 1948511, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2009年 / 146卷 / 03期

关键词：

DNA helicase; DNA replication; RecQ helicase; ATPase; Rothmund-Thomson syndrome; ROTHMUND-THOMSON-SYNDROME; STRAND-ANNEALING ACTIVITIES; HELA-CELL NUCLEI; UNWINDING ACTIVITY; REPLICATION; INVOLVEMENT; INITIATION; PRODUCT; COMPLEX; SYSTEM;

D O I：

10.1093/jb/mvp074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human RECQL4 protein was expressed in insect cells using a baculovirus protein expression system and it was purified to near homogeneity. The protein sedimented at a position between catalase (230 kDa) and ferritin (440 kDa) in glycerol gradient centrifugation, suggesting that it forms homo-multimers. Activity to displace annealed 17-mer oligonucleotide in the presence of ATP was co-sedimented with hRECQL4 protein. In ion-exchange chromatography, both DNA helicase activity and single-stranded DNA-dependent ATPase activity were co-eluted with hRECQL4 protein. The requirements of ATP and Mg for the helicase activity were different from those for the ATPase activity. The data suggest that the helicase migrates on single-stranded DNA in a 3'-5' direction. These results suggest that the hRECQL4 protein exhibits DNA helicase activity.

引用

页码：327 / 335

页数：9

共 50 条

[1] RAPADILINO RECQL4 mutant protein lacks helicase and ATPase activity
Croteau, Deborah L.
Rossi, Marie L.
Ross, Jennifer
Dawut, Lale
Dunn, Christopher
Kulikowicz, Tomasz
Bohr, Vilhelm A.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2012, 1822 (11): : 1727 - 1734
[2] ATM activation is impaired in human cells defective in RecQL4 helicase activity
Park, Soon-Young
Kim, Hyunsup
Im, Jun-Sub
Lee, Joon-Kyu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 509 (02) : 379 - 383
[3] RecQL4 Helicase Amplification Is Involved in Human Breast Tumorigenesis
Fang, Hongbo
Nie, Linghu
Chi, Zhenfen
Liu, Jing
Guo, Dan
Lu, Xuemei
Hei, Tom K.
Balajee, Adayabalam S.
Zhao, Yongliang
PLOS ONE, 2013, 8 (07):
[4] Human RecQL4 Helicase Plays Critical Roles in Prostate Carcinogenesis
Su, Yanrong
Meador, Jarah A.
Calaf, Gloria M.
De-Santis, Luca Proietti
Zhao, Yongliang
Bohr, Vilhelm A.
Balajee, Adayabalam S.
CANCER RESEARCH, 2010, 70 (22) : 9207 - 9217
[5] The DNA Helicase Recql4 Is Required for Normal Osteoblast Expansion and Osteosarcoma Formation
Ng, Alvin J. M.
Walia, Mannu K.
Smeets, Monique F.
Mutsaers, Anthony J.
Sims, Natalie A.
Purton, Louise E.
Walsh, Nicole C.
Martin, T. John
Walkley, Carl R.
PLOS GENETICS, 2015, 11 (04):
[6] ATP-dependent helicase activity is dispensable for the physiological functions of Recql4
Castillo-Tandazo, Wilson
Smeets, Monique F.
Murphy, Vincent
Liu, Rui
Hodson, Charlotte
Heierhorst, Joerg
Deans, Andrew J.
Walkley, Carl R.
PLOS GENETICS, 2019, 15 (07):
[7] The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif
Ohlenschlaeger, Oliver
Kuhnert, Anja
Schneider, Annerose
Haumann, Sebastian
Bellstedt, Peter
Keller, Heidi
Saluz, Hans-Peter
Hortschansky, Peter
Haenel, Frank
Grosse, Frank
Goerlach, Matthias
Pospiech, Helmut
NUCLEIC ACIDS RESEARCH, 2012, 40 (17) : 8309 - 8324
[8] RECQL4 is not critical for firing of human DNA replication origins
Padayachy, Laura
Ntallis, Sotirios G.
Halazonetis, Thanos D.
SCIENTIFIC REPORTS, 2024, 14 (01):
[9] PROTEIN TRUNCATING, BUT NOT HELICASE INACTIVATING, RECQL4 MUTATIONS CAUSE BONE MARROW FAILURE
Castillo-Tandazo, Wilson
Smeets, Monique
Walkley, Carl
EXPERIMENTAL HEMATOLOGY, 2020, 88 : S56 - S56
[10] Analysis of the RECQL4 helicase gene in patients with dyskeratosis congenita
Walne, A. J.
Marrone, A.
Beswick, R.
Kirwan, M.
Dokal, I.
Vulliamy, T.
BRITISH JOURNAL OF HAEMATOLOGY, 2008, 141 : 63 - 63

← 1 2 3 4 5 →