Comprehensive Analysis Reveals Novel Interactions between Circulating MicroRNAs and Gut Microbiota Composition in Human Obesity

被引:31
作者
Assmann, Tais Silveira [1 ]
Cuevas-Sierra, Amanda [1 ]
Riezu-Boj, Jose Ignacio [1 ,2 ,3 ]
Milagro, Fermin I. [1 ,2 ,3 ]
Martinez, J. Alfredo [1 ,2 ,3 ]
机构
[1] Univ Navarra, Ctr Nutr Res, Dept Nutr Food Sci & Physiol, Irunlarrea 1, Pamplona 31008, Spain
[2] Carlos III Hlth Inst, Ctr Invest Biomed Red Fisiopatol Obesidad & Nutr, Madrid 28029, Spain
[3] IdiSNA Navarra Inst Hlth Res, Pamplona 31008, Spain
关键词
BMI; miRNA; obesity; Bacteroides eggerthi; gut microbiota; PHYSICAL-ACTIVITY; NONCODING RNAS; PLASMA; SIGNATURE; PREDICTION; BIOMARKERS; PATHWAY; PROFILE; WEIGHT;
D O I
10.3390/ijms21249509
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: The determinants that mediate the interactions between microRNAs and the gut microbiome impacting on obesity are scarcely understood. Thus, the aim of this study was to investigate possible interactions between circulating microRNAs and gut microbiota composition in obesity. Method: The sample comprised 78 subjects with obesity (cases, body mass index (BMI): 30-40 kg/m(2)) and 25 eutrophic individuals (controls, BMI <= 25 kg/m(2)). The expression of 96 microRNAs was investigated in plasma of all individuals using miRCURY LNA miRNA Custom PCR Panels. Bacterial DNA sequencing was performed following the Illumina 16S protocol. The FDR correction was used for multiple comparison analyses. Results: A total of 26 circulating microRNAs and 12 bacterial species were found differentially expressed between cases and controls. Interestingly, an interaction among three miRNAs (miR-130b-3p, miR-185-5p and miR-21-5p) with Bacteroides eggerthi and BMI levels was evidenced (r(2) = 0.148, p = 0.004). Moreover, these microRNAs regulate genes that participate in metabolism-related pathways, including fatty acid degradation, insulin signaling and glycerolipid metabolism. Conclusions: This study characterized an interaction between the abundance of 4 bacterial species and 14 circulating microRNAs in relation to obesity. Moreover, the current study also suggests that miRNAs may serve as a communication mechanism between the gut microbiome and human hosts.
引用
收藏
页码:1 / 17
页数:17
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