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Age-related changes in the ductular system and stellate cells of human pancreas
被引:1
|作者:
Saini, Shubhi
[1
]
Jacob, Tony George
[1
]
Bhardwaj, D. N.
[2
]
Roy, Tara Sankar
[1
]
机构:
[1] All India Inst Med Sci, Dept Anat, New Delhi 110029, India
[2] All India Inst Med Sci, Dept Forens Med, New Delhi, India
关键词:
Pancreatic ducts;
Aging;
Fibrosis;
Immunohistochemistry;
Chronic pancreatitis;
FIBROSIS-RELATED GENES;
COLLAGEN DEPOSITION;
EXPRESSION;
CANCER;
TISSUE;
DUCTS;
D O I:
10.1016/j.jasi.2015.10.011
中图分类号:
R602 [外科病理学、解剖学];
R32 [人体形态学];
学科分类号:
100101 ;
摘要:
Introduction: Age associated progressive fibrosis may be a major causative factor that leads to pathogenesis of many diseases. Activated pancreatic stellate cells (alpha-SMA positive) play a major role in fibrogenesis that affects the cytoarchitecture and functioning of pancreas. This study dealt with age-related fibrotic changes in the ductular system of the tail and body of pancreas and the morphology of pancreatic stellate cells. Methods: Pancreata (n = 36) from cadavers aged 30-80 years were obtained after due clearances and processed for Masson's trichome staining. Fibrosis was quantified using Adobe Photoshop (CS2) and Image-J software. Hierarchical cluster analysis was done on the luminal area and total ductal area that were measured by the nucleator probe of StereoInvestigator software (MBF, Vermont, USA). Pancreatic stellate cells (alpha-SMA positive cells) were identified by immunohistochemistry and quantified stereologically around periacinar, periductular, perivascular, and peri-Islet areas. Results: An increased fibrosis was noted in body and tail regions of the pancreas with increasing age. Three duct populations were identified in clustering. Their area and corresponding lumen showed a significant increase with progressive decades (p < 0.001). alpha-SMA positive cells increased significantly from 4th to 7th decades (p = 0.002, 0.004 and 0.002, respectively). Discussion: Pancreatic stellate cells may be important contributors to increased fibrosis in pancreas. The classification of pancreatic ducts into three clusters may serve to be a useful tool. (C) 2015 Published by Elsevier, a division of Reed Elsevier India, Pvt. Ltd on behalf of Anatomical Society of India.
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页码:99 / 106
页数:8
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