A new proteinaceous pathogen-associated molecular pattern (PAMP) identified in Ascomycete fungi induces cell death in Solanaceae

被引:52
作者
Franco-Orozco, Barbara [1 ]
Berepiki, Adokiye [1 ]
Ruiz, Olaya [2 ]
Gamble, Louise [1 ]
Griffe, Lucie L. [1 ]
Wang, Shumei [1 ]
Birch, Paul R. J. [3 ]
Kanyuka, Kostya [2 ]
Avrova, Anna [1 ]
机构
[1] James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, Scotland
[2] Rothamsted Res, Plant Biol & Crop Sci Dept, Harpenden AL5 2JQ, Herts, England
[3] Univ Dundee JHI, Div Plant Sci, Dundee DD2 5DA, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
Ascomycete; cell death; elicitor; fungal; pathogen; pathogen-associated molecular pattern (PAMP); Rhynchosporium commune; Solanaceae; PHYTOPHTHORA-INFESTANS; DISEASE RESISTANCE; INNATE IMMUNITY; INTERFAMILY TRANSFER; CELLULOSE-BINDING; CHITIN FRAGMENTS; CERATO-PLATANIN; PLASMA-MEMBRANE; RECEPTOR KINASE; MAPKKK-EPSILON;
D O I
10.1111/nph.14542
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pathogen-associated molecular patterns (PAMPs) are detected by plant pattern recognition receptors (PRRs), which gives rise to PAMP-triggered immunity (PTI). We characterized a novel fungal PAMP, Cell Death Inducing 1 (RcCDI1), identified in the Rhynchosporium commune transcriptome sampled at an early stage of barley (Hordeum vulgare) infection. The ability of RcCDI1 and its homologues from different fungal species to induce cell death in Nicotiana benthamiana was tested following agroinfiltration or infiltration of recombinant proteins produced by Pichia pastoris. Virus-induced gene silencing (VIGS) and transient expression of Phytophthora infestans effectors PiAVR3a and PexRD2 were used to assess the involvement of known components of PTI in N. benthamiana responses to RcCDI1. RcCDI1 was highly upregulated early during barley colonization with R. commune. RcCDI1 and its homologues from different fungal species, including Zymoseptoria tritici, Magnaporthe oryzae and Neurospora crassa, exhibited PAMP activity, inducing cell death in Solanaceae but not in other families of dicots or monocots. RcCDI1-triggered cell death was shown to require N. benthamiana Brassinosteroid insensitive 1-Associated Kinase 1 (NbBAK1), N. benthamiana suppressor of BIR1-1 (NbSOBIR1) and N. benthamiana SGT1 (NbSGT1), but was not suppressed by PiAVR3a or PexRD2. We report the identification of a novel Ascomycete PAMP, RcCDI1, recognized by Solanaceae but not by monocots, which activates cell death through a pathway that is distinct from that triggered by the oomycete PAMP INF1.
引用
收藏
页码:1657 / 1672
页数:16
相关论文
共 83 条
[1]  
Albert I, 2015, NAT PLANTS, V1, DOI [10.1038/NPLANTS.2015.140, 10.1038/nplants.2015.140]
[2]   Regulatory role of SGT1 in early R gene-mediated plant defenses [J].
Austin, MJ ;
Muskett, P ;
Kahn, K ;
Feys, BJ ;
Jones, JDG ;
Parker, JE .
SCIENCE, 2002, 295 (5562) :2077-2080
[3]   Rhynchosporium commune: a persistent threat to barley cultivation [J].
Avrova, Anna ;
Knogge, Wolfgang .
MOLECULAR PLANT PATHOLOGY, 2012, 13 (09) :986-997
[4]   Profiling and quantifying differential gene transcription in Phytophthora infestans prior to and during the early stages of potato infection [J].
Avrova, AO ;
Venter, E ;
Birch, PRJ ;
Whisson, SC .
FUNGAL GENETICS AND BIOLOGY, 2003, 40 (01) :4-14
[5]   The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance [J].
Azevedo, C ;
Sadanandom, A ;
Kitagawa, K ;
Freialdenhoven, A ;
Shirasu, K ;
Schulze-Lefert, P .
SCIENCE, 2002, 295 (5562) :2073-2076
[6]   Developmental expression of stress response genes in Theobroma cacao leaves and their response to Nep1 treatment and a compatible infection by Phytophthora megakarya [J].
Bailey, BA ;
Bae, H ;
Strem, MD ;
de Mayolo, GA ;
Guiltinan, MJ ;
Verica, JA ;
Maximova, SN ;
Bowers, JH .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2005, 43 (06) :611-622
[7]   AN ETHYLENE BIOSYNTHESIS-INDUCING ENDOXYLANASE ELICITS ELECTROLYTE LEAKAGE AND NECROSIS IN NICOTIANA-TABACUM CV XANTHI LEAVES [J].
BAILEY, BA ;
DEAN, JFD ;
ANDERSON, JD .
PLANT PHYSIOLOGY, 1990, 94 (04) :1849-1854
[8]   A Conserved Peptide Pattern from a Widespread Microbial Virulence Factor Triggers Pattern-Induced Immunity in Arabidopsis [J].
Boehm, Hannah ;
Albert, Isabell ;
Oome, Stan ;
Raaymakers, Tom M. ;
Van den Ackerveken, Guido ;
Nuernberger, Thorsten .
PLOS PATHOGENS, 2014, 10 (11)
[9]  
BOLLER T, 1995, ANNU REV PLANT PHYS, V46, P189, DOI 10.1146/annurev.arplant.46.1.189
[10]   A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors [J].
Boller, Thomas ;
Felix, Georg .
ANNUAL REVIEW OF PLANT BIOLOGY, 2009, 60 :379-406