Plasticizer DBP Activates NLRP3 Inflammasome through the P2X7 Receptor in HepG2 and L02 Cells

被引：26

作者：

Ni, Jiahua ^{[1
]}

Zhang, Zihui ^{[1
]}

Luo, Xiaoqin ^{[1
]}

Xiao, Lei ^{[1
]}

Wang, Nanping ^{[2
]}

机构：

[1] Xi An Jiao Tong Univ, Cardiovasc Res Ctr, Xian 710061, Peoples R China

[2] Dalian Med Univ, Adv Inst Med Sci, Dalian 116044, Peoples R China

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2016年 / 30卷 / 04期

基金：

美国国家科学基金会;

关键词：

Plasticizer; DBP; NLRP3; Inflammasome; P2X(7) Receptor; NALP3; INFLAMMASOME; PROINFLAMMATORY CYTOKINES; PHTHALATE-ESTERS; RELEASE; LIVER; DEHP; MACROPHAGES; METABOLITES; SECRETION; MOLECULES;

D O I：

10.1002/jbt.21776

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ditubyl phthalate (DBP), one of the most widely used plasticizers, can migrate out to contaminate our bodies and environment. A number of studies have showed that DBP is closely related to liver pathological changes and diseases. Inflammasomes are multiprotein complexes composed of procaspase and pattern recognition receptors such as Nucleotide oligomerization domain (NOD) like receptor family, pyrin domain containing 3 (NLRP3). Activation of NLRP3 inflammasome is implicated in the pathogeneses of liver damage. The aim of this study was to determine the effects of DBP on NLRP3 inflammasome. We found that DBP triggered the activation of NLRP3 inflammasome in hepatocyte cell lines. By using Ca-074-Me, N-acetylcysteine and KN-62, we observed that the P2X(7) receptor participated in the DBP-induced activation of NLRP3 inflammasome. DBP could also trigger the ATP release. In conclusion, we demonstrated that DBP is one of the activator of NLRP3 inflammasome and may play an important role in liver damage.

引用

页码：178 / 185

页数：8

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