Rapid selection of peptide containing fractions in off-line 2-D HPLC in shotgun proteome analysis by screening with MALDI TOF MS

被引：2
|
作者：
Lasaosa, Maria
Jakoby, Thomas [1 ,3 ]
Delmotte, Nathanael
Huber, Christian G. [2 ]
Heinzle, Elmar
Tholey, Andreas [1 ]
机构：
[1] Univ Kiel, Div Systemat Proteome Res, Inst Expt Med, D-24105 Kiel, Germany
[2] Salzburg Univ, Dept Mol Biol, Div Chem & Bioanalyt, A-5020 Salzburg, Austria
[3] Univ Saarland, Ctr Bioinformat, Jr Res Grp Prot Prot Interact & Computat Prote, Saarbrucken, Germany
来源：
PROTEOMICS | 2009年 / 9卷 / 19期
关键词：
DESORPTION/IONIZATION MASS-SPECTROMETRY; SEPARATION; IDENTIFICATION; DIMENSIONS; PH;
D O I：
10.1002/pmic.200900274
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and fast screening method for the selection of fractions of first dimension separation to be analyzed in second dimension-MS/MS experiments in offline multidimensional liquid chromatographic separation schemes for shotgun proteome analysis was developed. The method is based on the measurement of total peptide content of the first dimension fractions by MALDI MS and was established using a tryptic digest of a bacterial proteome. The results of the screening process were in good agreement with those obtained in a detailed proteome analysis performed by RP x ion-pair RP-MALDI TOF/TOF MS analysis The method supports a straightforward planning of experiments, also enabling a reduction of overall measurement time in shotgun proteome analysis.
引用
收藏
页码：4577 / 4581
页数：5
相关论文
共 23 条
  • [21] Rapid screening and identification of antioxidants in the leaves of Malus hupehensis using off-line two-dimensional HPLC-UV-MS/MS coupled with a 1,1′-diphenyl-2-picrylhydrazyl assay
    Liu, Minzhuo
    Huang, Xueqian
    Liu, Qi
    Chen, Miao
    Liao, Sen
    Zhu, Fawei
    Shi, Shuyun
    Yang, Hua
    Chen, Xiaoqing
    JOURNAL OF SEPARATION SCIENCE, 2018, 41 (12) : 2536 - 2543
  • [22] INSTANT DETERMINATION OF CARDIAC-OUTPUT USING AUTOMATED ACOUSTIC QUANTIFICATION APPROACH IN CRITICALLY ILL PATIENTS - COMPARISON WITH THERMODILUTION METHOD AND OFF-LINE 2-D ECHOCARDIOGRAPHIC ANALYSIS
    TARDIF, JC
    CAO, QL
    ESAKOFF, D
    POLLARD, H
    SCHWARTZ, S
    CIRCULATION, 1993, 88 (04) : 218 - 218
  • [23] Proteome analysis of light-induced proteins in Synechocystis sp PCC 6803:: Identification of proteins separated by 2D-PAGE using N-terminal sequencing and MALDI-TOF MS
    Choi, JS
    Kim, DS
    Lee, J
    Kim, SJ
    Kim, SI
    Kim, YH
    Hong, J
    Yoo, JS
    Suh, KH
    Park, YM
    MOLECULES AND CELLS, 2000, 10 (06) : 705 - 711
123