Rapid selection of peptide containing fractions in off-line 2-D HPLC in shotgun proteome analysis by screening with MALDI TOF MS

被引：2
|
作者：
Lasaosa, Maria
Jakoby, Thomas [1 ,3 ]
Delmotte, Nathanael
Huber, Christian G. [2 ]
Heinzle, Elmar
Tholey, Andreas [1 ]
机构：
[1] Univ Kiel, Div Systemat Proteome Res, Inst Expt Med, D-24105 Kiel, Germany
[2] Salzburg Univ, Dept Mol Biol, Div Chem & Bioanalyt, A-5020 Salzburg, Austria
[3] Univ Saarland, Ctr Bioinformat, Jr Res Grp Prot Prot Interact & Computat Prote, Saarbrucken, Germany
来源：
PROTEOMICS | 2009年 / 9卷 / 19期
关键词：
DESORPTION/IONIZATION MASS-SPECTROMETRY; SEPARATION; IDENTIFICATION; DIMENSIONS; PH;
D O I：
10.1002/pmic.200900274
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and fast screening method for the selection of fractions of first dimension separation to be analyzed in second dimension-MS/MS experiments in offline multidimensional liquid chromatographic separation schemes for shotgun proteome analysis was developed. The method is based on the measurement of total peptide content of the first dimension fractions by MALDI MS and was established using a tryptic digest of a bacterial proteome. The results of the screening process were in good agreement with those obtained in a detailed proteome analysis performed by RP x ion-pair RP-MALDI TOF/TOF MS analysis The method supports a straightforward planning of experiments, also enabling a reduction of overall measurement time in shotgun proteome analysis.
引用
收藏
页码：4577 / 4581
页数：5
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