Role of Bifidobacterium longum in the induction of apoptotic deletion in the human enterocyte-like Caco-2 cell line

被引:2
作者
Nissen, Lorenzo
Pasini, Luca
Biavati, Bruno
Malagolini, Nadia
Dall'Olio, Fabio
Della Valle, Giuliano
Sgorbati, Barbara [1 ]
机构
[1] Univ Bologna, Alma Mater Studiorum, Dipartimento Sci & Tecnol Agroambientali, Bologna, Italy
[2] Univ Bologna, Alma Mater Studiorum, Dipartimento Biol Evoluzionist Sperimentale, Bologna, Italy
[3] Univ Bologna, Alma Mater Studiorum, Dipartimento Patol Sperimentale, Bologna, Italy
关键词
probiotics; adhesion; apoptotic deletion; restoring ecology;
D O I
10.1007/BF03175024
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bifidobacterium longum is a probiotic, known for its beneficial effects to the human gut and even for its immunomodulatory and antitumor activities. Recently, many studies have stressed out the intimate relation between probiotic bacteria and the GIT mucosa and their influence on human cellular homeostasis. We focused on the apoptotic deletion of cancer cells induced by B. longum. This has been valued in vitro, performing the incubation of three B. longum strains with enterocyte-like Caco-2 cells, to evidence DNA fragmentation, a cornerstone of apoptosis. The three strains tested were defined for their adhesion properties using adhesion and autoaggregation assays. These features are considered necessary to select a probiotic strain. The three strains named B12, B18 and B2990 resulted respectively: "strong adherent", "adherent" and "non adherent". Then, bacteria were incubated with Caco-2 cells to investigate apoptotic deletion. Cocultures of Caco-2 cells with B. longum resulted positive in DNA fragmentation test, only when adherent strains were used (B12 and B18). These results indicate that the interaction with adherent B. longum can induce apoptotic deletion of Caco-2 cells, suggesting a role in cellular homeostasis of the gastrointestinal tract and in restoring the ecology of damaged colon tissues.
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页码:319 / 323
页数:5
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