Generating ventral spinal organoids from human induced pluripotent stem cells

被引：15

作者：

Hor, Jin-Hui ^{[1
,2
]}

Ng, Shi-Yan ^{[1
,3
,4
,5
]}

机构：

[1] A STAR Res Entities, Inst Mol & Cell Biol, Singapore, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med Physiol, Singapore, Singapore

[4] Guangzhou Med Univ, Affiliated Hosp 3, Guangzhou, Peoples R China

[5] Natl Neurosci Inst, Singapore, Singapore

来源：

HUMAN PLURIPOTENT STEM CELL DERIVED ORGANOID MODELS | 2020年 / 159卷

关键词：

NEURONAL SPECIFICATION; MOTOR-NEURONS; MOUSE MODEL; CORD; DIFFERENTIATION; INTERNEURONS; IDENTITY; DISTINCT; SIGNALS; GDF11;

D O I：

10.1016/bs.mcb.2020.03.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Current advances in human pluripotent stem cell (hPSC) technology allow directed differentiation into three-dimensional spinal organoid cultures that mimic the unique microenvironment and cytoarchitecture of the human spinal cord. Organoids also serves as important cellular tools to model spinal cord development and motor neuron diseases such as Spinal Muscular Atrophy and Amyotrophic Lateral Sclerosis. In this chapter, we describe a detailed step-by-step methodology to generate spinal organoids from human PSCs. We include advantages and limitations of the use of spinal organoids and notes on technical and design considerations in generating these spinal organoids.

引用

页码：257 / 277

页数：21

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