Genotypic variation in anther culture and effect of ovary coculture in durum wheat

被引：16
作者：
J'Aiti, F
Benlhabib, O
Sharma, HC
El Jaafari, S
El Hadrami, I
机构：
[1] Univ Cadi Ayyad, Dept Biol, Lab Plant Physiol, Fac Sci Semlalia, Marrakech 40001, Morocco
[2] IAV Hassan II, Dept Agron, Rabat, Morocco
[3] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA
[4] Fac Sci, Lab Biotechnol & Ameliorat Plantes, Meknes 50000, Morocco
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1999年 / 59卷 / 01期
基金：
加拿大魁北克医学研究基金会;
关键词：
androgenic potential; anther culture; anther-ovary coculture; durum wheat;
D O I：
10.1023/A:1006399512217
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed.
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收藏
页码：71 / 76
页数：6
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