Back to the roots: protocol for the photoautotrophic micropropagation of medicinal Cannabis

被引：29

作者：

Kodym, Andrea ^{[1
,2
]}

Leeb, Christian J. ^{[1
]}

机构：

[1] Univ Vienna, Dept Pharmacognosy, A-1090 Vienna, Austria

[2] Univ Vienna, Core Facil Bot Garden, A-1030 Vienna, Austria

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2019年 / 138卷 / 02期

基金：

奥地利科学基金会;

关键词：

Aseptic culture; In vitro; Cannabis sativa; Plant tissue culture; Vegetative propagation;

D O I：

10.1007/s11240-019-01635-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this protocol was to develop an alternative in vitro propagation system for Cannabis sativa L. by mimicking nursery-based vegetative propagation. Photoautotrophic micropropagation (PAM) was achieved on rockwool blocks as substrate combined with commercially available fertilizer suitable for cannabis cultivation. Stock plants were initiated after sterilisation in forced-ventilated glass jars which then provided a continuous supply of shoot tip and nodal cuttings. A 97.5% rooting rate of in vitro shoot tip cuttings and successful acclimatisation were achieved within 3 weeks in glass vessels with passive ventilation. Key messageCannabis is a difficult plant to grow in vitro, in particular habit is strongly influenced by culture conditions. A photo-autotropic micropropagation system was developed to overcome culture-induced problems.

引用

页码：399 / 402

页数：4

共 9 条

[1]

Casano S., 2009, Italus Hortus, V16, P109

[2]

Guerriero G, 2016, FRONT PLANT SCI, V7, DOI [10.3389/fpls.2016.00019, 10.3389/fpls.2016.00463]

[3]

INCB, 2018, NARC DRUGS 2018

[4]

Lata H, 2016, J APPL RES MED AROMA

[5]

Lata H., 2017, Cannabis sativa L. - Botany and Biotechnology, P285, DOI [DOI 10.1007/978-3-319-54564-6_13, 10.1007/978-3-319-54564-6, DOI 10.1007/978-3-319-54564-6]

[6]

Lata H, 2016, METHODS MOL BIOL, V1391, P275, DOI 10.1007/978-1-4939-3332-7_19

[7]

Pierik R.L. M., 1997, In Vitro Culture of Higher Plants, P231

[8]

Nguyen QT, 2016, PLANT FACTORY: AN INDOOR VERTICAL FARMING SYSTEM FOR EFFICIENT QUALITY FOOD PRODUCTION, P271, DOI 10.1016/B978-0-12-801775-3.00020-2

[9]

Wang R, 2009, PAK J BOT

← 1 →