Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

被引：44

作者：

Ferroni, A

Sermet-Gaudelus, I

Abachin, E

Quesne, G

Lenoir, G

Berche, P

Gaillard, JL

机构：

[1] Hop Necker Enfants Malad, Microbiol Lab, F-75015 Paris, France

[2] Hop Necker Enfants Malad, Dept Pediat, F-75015 Paris, France

[3] Hop Ray Poincare, Microbiol Lab, Garches, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期

关键词：

D O I：

10.1128/JCM.40.10.3793-3797.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (I isolate), Burkholderia gladioli (I isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

引用

收藏

页码：3793 / 3797

页数：5

相关论文

共 50 条

[31] Activity of disinfectants against Gram-negative bacilli isolated from patients undergoing lung transplantation for cystic fibrosis [J].

Perry, JD ;

Riley, G ;

Johnston, S ;

Dark, JH ;

Gould, FK .

JOURNAL OF HEART AND LUNG TRANSPLANTATION, 2002, 21 (11) :1230-1231

[32] Prevalence of 16S rRNA methylases in Gram-negative bacteria derived from companion animals and livestock in Japan [J].

Usui, Masaru ;

Kajino, Akari ;

Kon, Michiha ;

Fukuda, Akira ;

Sato, Tomomi ;

Shirakawa, Takahiro ;

Kawanishi, Michiko ;

Harada, Kazuki ;

Nakajima, Chie ;

Suzuki, Yasuhiko ;

Tamura, Yutaka .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 2019, 81 (06) :874-878

[33] MOLECULAR IDENTIFICATION OF CLINICAL MICROBES IN THALASSEMIA PATIENTS USING 16S rRNA GENE SEQUENCING [J].

Pishtiwan, A. H. ;

Khadija, Kh M. .

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, 2019, 17 (06) :13135-13146

[34] 16S rRNA analysis of isolates obtained from gram-negative, filamentous bacteria micromanipulated from activated sludge [J].

Bradford, D ;

Hugenholtz, P ;

Seviour, EM ;

Cunningham, MA ;

Stratton, H ;

Seviour, RJ ;

Blackall, LL .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (03) :334-343

[35] Clonal diversity, antimicrobial resistance, and genome features among nonfermenting gram-negative bacteria isolated from patients with cystic fibrosis in Russia [J].

Bocharova, Yuliya ;

Chebotar, Igor ;

Savinova, Tatiana ;

Lyamin, Artem ;

Kondratenko, Olga ;

Polikarpova, Svetlana ;

Fedorova, Natalia ;

Semykin, Sergey ;

Korostin, Dmitriy ;

Chaplin, Andrey ;

Shagin, Dmitriy ;

Mayanskiy, Nikolay .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2024, 108 (02)

[36] In vitro activity of doripenem (S-4661) against multidrug-resistant gram-negative bacilli isolated from patients with cystic fibrosis [J].

Chen, YH ;

Garber, E ;

Zhao, QQ ;

Ge, YG ;

Wikler, MA ;

Kaniga, K ;

Saiman, L .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (06) :2510-2511

[37] Identification of Burkholderia and Uncommon Glucose-Nonfermenting Gram-Negative Bacilli Isolated from Patients with Cystic Fibrosis by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) [J].

Plongla, Rongpong ;

Panagea, Theofano ;

Pincus, David H. ;

Jones, Melissa C. ;

Gilligan, Peter H. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (12) :3071-3072

[38] 16S rRNA Sequencing as a Diagnostic Tool in the Identification of Culture-Negative Endocarditis in Surgically Treated Patients [J].

Kumar, Naveen, V ;

Menon, Thangam ;

Pathipati, Padmaja ;

Cherian, Kotturathu Mammen .

JOURNAL OF HEART VALVE DISEASE, 2013, 22 (06) :846-849

[39] 16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures [J].

Justesen, Ulrik Stenz ;

Skov, Marianne Nielsine ;

Knudsen, Elisa ;

Holt, Hanne Marie ;

Sogaard, Per ;

Justesen, Tage .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) :946-948

[40] Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and b. mallei [J].

Gee, JE ;

Sacchi, CT ;

Glass, MB ;

De, BK ;

Weyant, RS ;

Levett, PN ;

Whitney, AM ;

Hoffmaster, AR ;

Popovic, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4647-4654

← 1 2 3 4 5 →