Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

被引：44

作者：

Ferroni, A

Sermet-Gaudelus, I

Abachin, E

Quesne, G

Lenoir, G

Berche, P

Gaillard, JL

机构：

[1] Hop Necker Enfants Malad, Microbiol Lab, F-75015 Paris, France

[2] Hop Necker Enfants Malad, Dept Pediat, F-75015 Paris, France

[3] Hop Ray Poincare, Microbiol Lab, Garches, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期

关键词：

D O I：

10.1128/JCM.40.10.3793-3797.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (I isolate), Burkholderia gladioli (I isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

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收藏

页码：3793 / 3797

页数：5

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