Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

被引：44

作者：

Ferroni, A

Sermet-Gaudelus, I

Abachin, E

Quesne, G

Lenoir, G

Berche, P

Gaillard, JL

机构：

[1] Hop Necker Enfants Malad, Microbiol Lab, F-75015 Paris, France

[2] Hop Necker Enfants Malad, Dept Pediat, F-75015 Paris, France

[3] Hop Ray Poincare, Microbiol Lab, Garches, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期

关键词：

D O I：

10.1128/JCM.40.10.3793-3797.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (I isolate), Burkholderia gladioli (I isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

引用

收藏

页码：3793 / 3797

页数：5

相关论文

共 50 条

[1] Comparison of Traditional Phenotypic Identification Methods with Partial 5′ 16S rRNA Gene Sequencing for Species-Level Identification of Nonfermenting Gram-Negative Bacilli [J].

Cloud, Joann L. ;

Harmsen, Dag ;

Iwen, Peter C. ;

Dunn, James J. ;

Hall, Gerri ;

LaSala, Paul Rocco ;

Hoggan, Karen ;

Wilson, Deborah ;

Woods, Gail L. ;

Mellmann, Alexander .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (04) :1442-1444

[2] Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis [J].

Kiska, DL ;

Kerr, A ;

Jones, M ;

Caracciolo, JA ;

Eskridge, B ;

Jordan, M ;

Miller, S ;

Hughes, D ;

King, N ;

Gilligan, PH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (04) :886-891

[3] Use of 16s rRNA gene sequencing for identification of "pseudomonas-like" bacilli isolated from sputum of patients with cystic fibrosis [J].

Moissenet, D ;

Arlet, G ;

Vu-Thien, H .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2004, 24 :S195-S196

[4] Capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of Pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis [J].

Ghozzi, R ;

Morand, P ;

Ferroni, A ;

Beretti, JL ;

Bingen, E ;

Segonds, C ;

Husson, MO ;

Izard, D ;

Berche, P ;

Gaillard, JL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3374-3379

[5] A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis [J].

da Costa Capizzani, Carolina Paulino ;

Cacador, Natalia Candido ;

Marques, Elizabeth Andrade ;

Levy, Carlos Emilio ;

Tonani, Ludmilla ;

Monteiro Marin Torres, Lidia Alice Gomes ;

da Costa Darini, Ana Lucia .

BRAZILIAN JOURNAL OF MICROBIOLOGY, 2018, 49 (02) :422-428

[6] Use of 16S rRNA gene sequencing for identification of "Pseudomonas-like" isolates from sputum of patients with cystic fibrosis [J].

Moissenet, D ;

Bingen, E ;

Arlet, G ;

Vu-Thien, H .

PATHOLOGIE BIOLOGIE, 2005, 53 (8-9) :500-502

[7] Evaluation of a Selection Strategy Before Use of 16S rRNA Gene Sequencing for the Identification of Clinically Significant Gram-Negative Rods and Coccobacilli [J].

Mahlen, Steven D. ;

Clarridge, Jill E., III .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2011, 136 (03) :381-388

[8] Identification of bacteria recovered from clinical specimens by 16S rRNA gene sequencing [J].

Kiratisin, P ;

Li, L ;

Murray, PR ;

Fischer, SH .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2003, 22 (10) :628-631

[9] Identification of Bacteria Recovered From Clinical Specimens by 16S rRNA Gene Sequencing [J].

P. Kiratisin ;

L. Li ;

P. R. Murray ;

S. H. Fischer .

European Journal of Clinical Microbiology and Infectious Diseases , 2003, 22 :628-631

[10] In silico analysis of 16S rRNA gene sequencing based methods for identification of medically important aerobic Gram-negative bacteria [J].

Teng, Jade L. L. ;

Yeung, Ming-Yiu ;

Yue, Geoffrey ;

Au-Yeung, Rex K. H. ;

Yeung, Eugene Y. H. ;

Fung, Ami M. Y. ;

Tse, Herman ;

Yuen, Kwok-Yung ;

Lau, Susanna K. P. ;

Woo, Patrick C. Y. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (09) :1281-1286

← 1 2 3 4 5 →