Fluorometric determination of microRNA using arched probe-mediated isothermal exponential amplification combined with DNA-templated silver nanoclusters

被引：22
作者：
Wu, Hao [1 ]
Wu, Jun [1 ]
Liu, Yaling [1 ]
Wang, Hongyong [1 ]
Zou, Pei [1 ]
机构：
[1] Jiangsu Inst Nucl Med, Minist Hlth, Jiangsu Key Lab Mol Nucl Med, Key Lab Nucl Med, Wuxi 214063, Jiangsu, Peoples R China
来源：
MICROCHIMICA ACTA | 2019年 / 186卷 / 11期
关键词：
MicroRNA-141; Arched probe; Silver nanoclusters; Isothermal amplification; DNA polymerase; Nicking endonuclease; Human serum; QUANTIFICATION;
D O I：
10.1007/s00604-019-3836-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly sensitive fluorometric method is described for the determination of microRNA-141. It is based on the use of arched probe-mediated isothermal exponential amplification reaction (EXPAR) and of DNA-templated silver nanoclusters (DNA-AgNCs). The EXPAR utilizes microRNA-141 as the trigger, polymerases and endonucleases as amplification activators, and two arched probes as exponential amplification templates. This enables the conversion of microRNA to a large number of reporter sequences under isothermal conditions within minutes. The generated reporter sequences act as scaffolds for the synthesis of fluorescent DNA-AgNCs by reduction of Ag (I) with NaBH4. The DNA-AgNCs function as signalling fluorophores with excitation/emission maxima at 540/610 nm. The method exhibits high sensitivity for microRNA-141 with a detection limit as low as 0.87 fM and a dynamic range from 1 fM to 500 fM. The method can distinguish nucleotides in the microRNA-200 family.
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页数：8
相关论文
共 33 条
[21]   DNA-templated silver nanoclusters: structural correlation and fluorescence modulation [J].
New, S. Y. ;
Lee, S. T. ;
Su, X. D. .
NANOSCALE, 2016, 8 (41) :17729-17746
[22]   Fluorescent silver nanoclusters as DNA probes [J].
Obliosca, Judy M. ;
Liu, Cong ;
Yeh, Hsin-Chih .
NANOSCALE, 2013, 5 (18) :8443-8461
[23]   Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example [J].
Reid, Michael S. ;
Le, X. Chris ;
Zhang, Hongquan .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (37) :11856-11866
[24]   Real-time PCR quantification of precursor and mature microRNA [J].
Schmittgen, Thomas D. ;
Lee, Eun Joo ;
Jiang, Jinmai ;
Sarkar, Anasuya ;
Yang, Liuqing ;
Elton, Terry S. ;
Chen, Caifu .
METHODS, 2008, 44 (01) :31-38
[25]   Impedimetric monitoring of apoptosis using cytochrome-aptamer bioconjugated silver nanocluster [J].
Shamsipur, Mojtaba ;
Pashabadi, Afshin ;
Molaabasi, Fatemeh ;
Hosseinkhani, Saman .
BIOSENSORS & BIOELECTRONICS, 2017, 90 :195-202
[26]   Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues [J].
Streit, Sylvia ;
Michalski, Christoph W. ;
Erkan, Mert ;
Kleeff, Joerg ;
Friess, Helmut .
NATURE PROTOCOLS, 2009, 4 (01) :37-43
[27]   Fluorometric determination of microRNA-122 by using ExoIII-aided recycling amplification and polythymine induced formation of copper nanoparticles [J].
Tang, Yafang ;
Liu, Mingxiu ;
Zhao, Zilin ;
Li, Qing ;
Liang, Xuehua ;
Tian, Jianniao ;
Zhao, Shulin .
MICROCHIMICA ACTA, 2019, 186 (03)
[28]   Molecular beacon immobilized on graphene oxide for enzyme-free signal amplification in electrochemiluminescent determination of microRNA [J].
Wang, Jiaxing ;
Zhang, Linlin ;
Lu, Liping ;
Kang, Tianfang .
MICROCHIMICA ACTA, 2019, 186 (03)
[29]   DNA-Stabilized Silver Nanoclusters for Label-Free Fluorescence Imaging of Cell Surface Glycans and Fluorescence Guided Photothermal Therapy [J].
Wu, Jing ;
Li, Na ;
Yao, Yao ;
Tang, Daoquan ;
Yang, Dongzhi ;
Machuki, Jeremiah Ong'achwa ;
Li, Jingjing ;
Yu, Yanyan ;
Gao, Fenglei .
ANALYTICAL CHEMISTRY, 2018, 90 (24) :14368-14375
[30]   Ultrasensitive Electrochemical Detection of MicroRNA Based on an Arched Probe Mediated Isothermal Exponential Amplification [J].
Yu, Yanyan ;
Chen, Zuanguang ;
Shi, Lijuan ;
Yang, Fan ;
Pan, Jianbin ;
Zhang, Beibei ;
Sun, Duanping .
ANALYTICAL CHEMISTRY, 2014, 86 (16) :8200-8205
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