Electrochemical Sensing of NADH and Glutamate Based on Meldola Blue in 1,2-Diaminobenzene and 3,4-Ethylenedioxythiophene Polymer Films

被引：19

作者：

Doaga, Rodica ^{[1
]}

McCormac, Timothy ^{[2
]}

Dempsey, Eithne ^{[1
]}

机构：

[1] Inst Technol Tallaght, Dept Sci, CREATE, Dublin 24, Ireland

[2] Dundalk Inst Technol, Electrochem Res Grp, Dundalk, County Louth, Ireland

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 19期

关键词：

Poly-1,2-diaminobenzene; Poly-3,4-ethylenedioxythiophene; Single walled carbon nanotubes; Meldola blue; Nicotinamide adenine dinucleotide; Glutamate; Nanotubes; Thin films; CARBON NANOTUBES; ELECTROCATALYTIC OXIDATION; ASCORBIC-ACID; BIOSENSOR; DEHYDROGENASE; POLY(O-PHENYLENEDIAMINE); ELECTRODES; COMPOSITE; CONDUCTIVITY; FABRICATION;

D O I：

10.1002/elan.200904627

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The redox mediator Meldola blue (MB) was entrapped into two polymers, poly-1,2-diaminobenzene (p-DAB) and poly-3,4-ethylenedioxythiophene (p-EDOT) by potential cycling and films were applied to NADH oxidation with subsequent glutamate detection using immobilized glutamate dehydrogenase. Both polymer films were tested for electrocatalysis of NADH using amperometry at E-app=0.1 V vs. Ag/AgCl and similar response characteristics were obtained with sensitivity values of 6.1 nA mu M-1, linear range up to 0.5 mM (R-2 = 0.9972) and LOD of 50 mu M. Subsequent amperometric determination of glutamate resulted in sensitivity 0.7 nA mu M-1, linearity 0-100 mu M and detection limit of 2 mu M glutamate.

引用

页码：2099 / 2108

页数：10

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