Interleukin-23 may contribute to the pathogenesis of lumbar disc herniation through the IL-23/IL-17 pathway

被引:24
作者
Jiang, Hongqiang [1 ]
Deng, Yao [2 ]
Wang, Tao [2 ]
Ma, Jianxiong [2 ]
Li, Pengfei [2 ]
Tian, Peng [2 ]
Han, Chao [2 ]
Ma, Xinlong [1 ,2 ]
机构
[1] Tianjin Med Univ, Gen Hosp, Dept Orthopaed, 154 Anshan Rd, Tianjin 300052, Peoples R China
[2] Tianjin Orthoped Inst, 155 Munan Rd, Tianjin 300050, Peoples R China
来源
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | 2016年 / 11卷
关键词
Lumbar disc herniation; Intervertebral disc; IL-23; IL-17; TUMOR-NECROSIS-FACTOR; AUTOLOGOUS NUCLEUS PULPOSUS; NERVE ROOT INJURY; LOW-BACK-PAIN; INTERVERTEBRAL DISC; CYTOKINE EXPRESSION; GROWTH-FACTOR; FACTOR-ALPHA; MATRIX METALLOPROTEINASES; PROSTAGLANDIN E(2);
D O I
10.1186/s13018-016-0343-8
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Background: Studies have indicated that interleukin 23 (IL-23) plays an important role in many inflammatory-and autoimmune-related diseases. However, there is little knowledge about IL-23 in lumbar disc herniation (LDH). Thus, in this study, we aimed to find out whether IL-23 is expressed in intervertebral discs (IVDs) and what roles it may play. Methods: Human IVD tissues were collected from 29 LDH patients and 8 vertebral fracture patients (normal control, NC group). According to the integrity of annulus fibrosus, LDH patients were divided into two groups: R group (ruptured group, n = 16) and NR group (non-ruptured group, n = 13). Morphological changes of IVDs were assessed by hematoxylin and eosin (HE staining), and expression of IL-23 in IVD tissues was detected by immunohistochemical staining. Besides gene expression of IL-23, IL-17, IL-6, IL-1 beta, and TNF-alpha was also evaluated by reverse transcription polymerase chain reaction (RT-PCR). Results: The results showed that the R group was more degenerated than the other two groups and NC group showed the least degenerated performance; stronger positive IL-23 expression was observed in herniated IVDs, especially in the R group. Meanwhile, higher gene expression of IL-23, IL-17, IL-6, IL-1 beta, and TNF-alpha was found in the tissues from LDH patients and a positive correlation between IL-17 and IL-23 gene expression was also observed. Conclusions: Taken all above results together, it may be deduced that higher expression of IL-23 may contribute to the deterioration of IVDs through the IL-23/IL-17 pathway.
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页数:8
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