Identification of a new broad-spectrum CD8+T cell epitope from over-expressed antigen COX-2 in esophageal carcinoma

被引：15

作者：

Gao, Yan-feng ^{[1
]}

Sun, Zhan-qiang ^{[1
]}

Qi, Feng ^{[1
]}

Qi, Yuan-ming ^{[1
]}

Zhai, Ming-xia ^{[1
]}

Lou, Hui-ping ^{[1
]}

Chen, Li-xiang ^{[1
]}

Li, Yong-xin ^{[1
]}

Wang, Xian-yuan ^{[2
]}

机构：

[1] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China

[2] Zhengzhou Univ, Dept Pathol, Affiliated Hosp 2, Zhengzhou 450001, Peoples R China

来源：

CANCER LETTERS | 2009年 / 284卷 / 01期

关键词：

Esophageal carcinoma; Epitope; Broad-spectrum; Cytotoxic T lymphocyte; Cyclooxygenase-2; CYTOTOXIC T-LYMPHOCYTES; MELANOMA-CELLS; MESSENGER-RNA; CANCER; POPULATION; TELOMERASE; BLOOD;

D O I：

10.1016/j.canlet.2009.04.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cyclooxygenase-2 (COX-2) has been found to be over-expressed in esophageal carcinoma (EC) and it could be considered as a potential tumor-associated antigen (TAA). In the present study, six candidate peptides from COX-2 were firstly predicted and synthesized. Among them, P-479 had the highest affinity and stability toward both HLA-A*0201 and HLA-A*03 molecules and it could significantly promote the IFN-gamma release. The cytotoxic T lymphocytes (CTLs) induced by P-479 could specifically lyse COX-2-expressed EC cell lines, EC-1 (HLA-A3 supertype) and EC-9706 (HLA-A2 supertype). These results suggested that P-479 as a novel broad-spectrum T cell epitope would be very useful in immunotherapy against esophageal carcinoma. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：55 / 61

页数：7

共 31 条

[1]

Chen SR, 2004, WORLD J GASTROENTERO, V10, P934

[2]

CORDONCARDO C, 1991, CANCER RES, V51, P6372

[3]

DUBOIS RN, 1994, AM J PHYSIOL, V266, P822

[4]

FERENC H, 2001, BIOLOGICALS, V29, P197

[5] Design of multi-epitope, analogue-based cancer vaccines [J].

Fikes, JD ;

Sette, A .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2003, 3 (06) :985-993

[6] Implications for immunosurveillance of altered HLA class I phenotypes in human tumours [J].

Garrido, F ;

RuizCabello, F ;

Cabrera, T ;

PerezVillar, JJ ;

LopezBotet, M ;

DugganKeen, M ;

Stern, PL .

IMMUNOLOGY TODAY, 1997, 18 (02) :89-95

[7] MHCPred: a server for quantitative prediction of peptide-MHC binding [J].

Guan, PP ;

Doytchinova, IA ;

Zygouri, C ;

Flower, DR .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3621-3624

[8] LIPOPOLYSACCHARIDE INDUCES PROSTAGLANDIN-H SYNTHASE-2 PROTEIN AND MESSENGER-RNA IN HUMAN ALVEOLAR MACROPHAGES AND BLOOD MONOCYTES [J].

HEMPEL, SL ;

MONICK, MM ;

HUNNINGHAKE, GW .

JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (01) :391-396

[9] Monitoring CD8 T cell responses to NY-ESO-1:: Correlation of humoral and cellular immune responses [J].

Jäger, E ;

Nagata, Y ;

Gnjatic, S ;

Wada, H ;

Stockert, E ;

Karbach, J ;

Dunbar, PR ;

Lee, SY ;

Jungbluth, A ;

Jäger, D ;

Arand, M ;

Ritter, C ;

Cerundolo, V ;

Dupont, B ;

Chen, YT ;

Old, LJ ;

Knuth, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (09) :4760-4765

[10]

KAGESHITA T, 1993, CANCER RES, V53, P3349

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