Production of Triterpenoid Sapogenins in Hairy Root Cultures of Silene vulgaris

被引：1

作者：

Kim, Yeon Bok ^{[1
,2
]}

Reed, Darwin W. ^{[2
]}

Covello, Patrick S. ^{[2
]}

机构：

[1] Chungnam Natl Univ, Dept Crop Sci, Taejon 305754, South Korea

[2] CNR, Saskatoon, SK S7N 0W9, Canada

来源：

NATURAL PRODUCT COMMUNICATIONS | 2015年 / 10卷 / 11期

关键词：

Agrobacterium rhizogenes; Hairy root culture; Methyl jasmonate; Triterpenoid saponins; Silene vulgaris; AGROBACTERIUM-TUMEFACIENS; ROL GENES; TRANSFORMATION; SAPONINS;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.

引用

页码：1919 / 1922

页数：4

共 18 条

[1] Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only [J].

Bonhomme, V ;

Laurain-Mattar, D ;

Lacoux, J ;

Fliniaux, MA ;

Jacquin-Dubreuil, A .

JOURNAL OF BIOTECHNOLOGY, 2000, 81 (2-3) :151-158

[2] GENETIC-TRANSFORMATION OF CATHARANTHUS-ROSEUS G DON BY AGROBACTERIUM-RHIZOGENES [J].

BRILLANCEAU, MH ;

DAVID, C ;

TEMPE, J .

PLANT CELL REPORTS, 1989, 8 (02) :63-66

[3]

Bruneton J., 1995, PHARMACOGNOSY PHYTOC, P538

[4]

Christey MC, 2005, METH MOL B, V286, P47

[5]

Dewick P.M., 2001, MED NATURAL PRODUCTS, V2nd

[6] Silenosides A-C, triterpenoid saponins from Silene vulgaris [J].

Glensk, M ;

Wray, V ;

Nimtz, M ;

Schöpke, T .

JOURNAL OF NATURAL PRODUCTS, 1999, 62 (05) :717-721

[7] Hairy root research:: recent scenario and exciting prospects -: Commentary [J].

Guillon, Stéphanie ;

Trémouillaux-Guiller, Jocelyne ;

Pati, Pratap Kumar ;

Rideau, Marc ;

Gantet, Pascal .

CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (03) :341-346

[8] Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng [J].

Han, Jung Yeon ;

Kwon, Yong Soo ;

Yang, Deok Chun ;

Jung, Young Rim ;

Choi, Yong Eui .

PLANT AND CELL PHYSIOLOGY, 2006, 47 (12) :1653-1662

[9]

Haralampidis K, 2002, ADV BIOCHEM ENG BIOT, V75, P31

[10] TRANSFER OF AGROBACTERIUM-TUMEFACIENS TI PLASMID TO AVIRULENT AGROBACTERIA AND TO RHIZOBIUM EX-PLANTA [J].

HOOYKAAS, PJJ ;

KLAPWIJK, PM ;

NUTI, MP ;

SCHILPEROORT, RA ;

RORSCH, A .

JOURNAL OF GENERAL MICROBIOLOGY, 1977, 98 (FEB) :477-484

← 1 2 →