Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study

被引：9

作者：

Rittipakorn, Pawornwan ^{[1
]}

Thuaksuban, Nuttawut ^{[1
]}

Mai-ngam, Katanchalee ^{[2
]}

Charoenla, Satrawut ^{[2
]}

Noppakunmongkolchai, Warobon ^{[2
]}

机构：

[1] Prince Songkla Univ, Fac Dent, Dept Oral & Maxillofacial Surg, Hat Yai 90110, Songkhla, Thailand

[2] Minist Higher Educ Sci Res & Innovat MHESRI, Bangkok 10400, Thailand

来源：

POLYMERS | 2021年 / 13卷 / 03期

关键词：

polycaprolactone; hydroxyapatite; BMP-2; scaffold; BONE MORPHOGENETIC PROTEIN-2; CALCIUM-PHOSPHATE SCAFFOLDS; MESENCHYMAL STEM-CELLS; GROWTH-FACTOR; DELIVERY; BIOCOMPATIBILITY; DIFFERENTIATION; EXPRESSION; DEFECTS; RHBMP-2;

D O I：

10.3390/polym13030466

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 mu g/mL bone morphogenetic protein-2 (BMP-2) solution prior to being seeded with osteoblasts, and they were cultured in the medium without BMP-2. In group B, the cell-scaffold constructs without BMP-2 were cultured in medium containing 10 mu g/mL BMP-2. The results showed greater cell proliferation in group A. The upregulation of runt-related transcription factor 2 and osteocalcin genes correlated with the release of BMP-2 from the scaffolds. The PCL-30% HA MSMD scaffolds appear to be suitable for use as osteoconductive frameworks and BMP-2 carriers.

引用

页码：1 / 15

页数：15

共 45 条

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