In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection

被引：23

作者：

Mitroulis, Ioannis ^{[1
]}

Kourtzelis, Ioannis ^{[1
]}

Papadopoulos, Vassileios P. ^{[1
]}

Mimidis, Konstantinos ^{[1
]}

Speletas, Matthaios ^{[2
]}

Ritis, Konstantinos ^{[1
]}

机构：

[1] Democritus Univ Thrace, Dept Internal Med 1, Alexandroupolis, Greece

[2] Univ Thessaly, Dept Immunol & Histocompatibil, Larisa, Greece

来源：

PARASITOLOGY INTERNATIONAL | 2009年 / 58卷 / 04期

关键词：

Leishmania donovani; Autophagy; Bone marrow macrophages; LC3B; TRYPANOSOMA-CRUZI; HOST-CELL; DIFFERENTIATION; MACROPHAGES; INHIBITION; PATHWAY; DISEASE;

D O I：

10.1016/j.parint.2009.07.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Autophagy is a homeostatic process promoting cell survival in periods of stress. The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani, which is the causative pathogen of visceral leishmaniasis, is an intracellular parasite that invades and multiplies in bone marrow macrophages. We describe the induction of host cell autophagic machinery during acute natural bone marrow infection by L. donovani complex, detected by LC3B immunoblot. The successful treatment with liposomal amphotericin B resulted in the resolution of this phenomenon. Even though the role of autophagy in parasite biology has been previously studied, our findings show for the first time the in vivo host cell LC3B conversion as a marker of the induction of the autophagic machinery during infection with Leishmania parasite in real time conditions. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：475 / 477

页数：3

共 17 条

[1] Autophagy is involved in nutritional stress response and differentiation in Trypanosoma cruzi
Alvarez, Vanina E.
Kosec, Gregor
Anna, Celso Sant
Turk, Vito
Cazzulo, Juan J.
Turk, Boris
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (06) : 3454 - 3464
[2] Endosome sorting and autophagy are essential for differentiation and virulence of Leishmania major
Besteiro, S
Williams, RAM
Morrison, LS
Coombs, GH
Mottram, JC
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (16) : 11384 - 11396
[3] MULTIPLICATION OF A HUMAN PARASITE (LEISHMANIA-DONOVANI) IN PHAGOLYSOSOMES OF HAMSTER MACROPHAGES INVITRO
CHANG, KP
DWYER, DM
[J]. SCIENCE, 1976, 193 (4254) : 678 - 680
[4] Autophagy: Many paths to the same end
Cuervo, AM
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2004, 263 (01) : 55 - 72
[5] Inhibition of phagolysosomal biogenesis by the Leishmania lipophosphoglycan
Desjardins, M
Descoteaux, A
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 185 (12) : 2061 - 2068
[6] Single-step duplex kDNA-PCR for detection of Leishmania donovani complex in human peripheral blood samples
Disch, Jolande
Caligiorne, Rachel B.
Maciel, Fabiana
Oliveira, Maria C.
Orsini, Mareela
Dias-Neto, Emmanuel
Rabello, Ana
[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 56 (04) : 395 - 400
[7] Cell biology - Autophagy as a regulated pathway of cellular degradation
Klionsky, DJ
Emr, SD
[J]. SCIENCE, 2000, 290 (5497) : 1717 - 1721
[8] Unveiling the roles of autophagy in innate and adaptive immunity
Levine, Beth
Deretic, Vojo
[J]. NATURE REVIEWS IMMUNOLOGY, 2007, 7 (10) : 767 - 777
[9] Methods for monitoring autophagy
Mizushima, N
[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (12) : 2491 - 2502
[10] Escape of intracellular Shigella from autophagy
Ogawa, M
Yoshimori, T
Suzuki, T
Sagara, H
Mizushima, N
Sasakawa, C
[J]. SCIENCE, 2005, 307 (5710) : 727 - 731

← 1 2 →