Albugo-imposed changes to tryptophanderived antimicrobial metabolite biosynthesis may contribute to suppression of non- host resistance to Phytophthora infestans in Arabidopsis thaliana

被引：28

作者：

Prince, David C. ^{[1
,2
]}

Rallapalli, Ghanasyam ^{[1
,3
]}

Xu, Deyang ^{[4
]}

Schoonbeek, Henk-Jan ^{[5
]}

Cevik, Volkan ^{[1
,6
]}

Asai, Shuta ^{[1
,7
]}

Kemen, Eric ^{[1
,8
]}

Cruz-Mireles, Neftaly ^{[1
]}

Kemen, Ariane ^{[1
,8
]}

Belhaj, Khaoula ^{[1
]}

Schornack, Sebastian ^{[1
,9
]}

Kamoun, Sophien ^{[1
]}

Holub, Eric B. ^{[10
]}

Halkier, Barbara A. ^{[4
]}

Jones, Jonathan D. G. ^{[1
]}

机构：

[1] Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[2] Univ East Anglia, Sch Biol Sci, Norwich Res Pk, Norwich, Norfolk, England

[3] Univ East Anglia, Norwich Med Sch, Norwich Res Pk, Norwich, Norfolk, England

[4] Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, DynaMo Ctr, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark

[5] John Innes Ctr, Dept Crop Genet, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[6] Univ Bath, Dept Biol & Biochem, Bath, Avon, England

[7] RIKEN, Yokohama Inst, Ctr Sustainable Resource Sci, Plant Immun Res Grp, Yokohama, Kanagawa, Japan

[8] Max Planck Inst Plant Breeding Res, Max Planck Res Grp Fungal Biodivers, Cologne, Germany

[9] Univ Cambridge, Sainsbury Lab, Cambridge, England

[10] Univ Warwick, Warwick Crop Ctr, Sch Life Sci, Wellesbourne, Warwick, England

来源：

BMC BIOLOGY | 2017年 / 15卷

基金：

英国生物技术与生命科学研究理事会; 新加坡国家研究基金会; 欧洲研究理事会; 日本学术振兴会;

关键词：

Phytophthora infestans; Albugo; Arabidopsis thaliana; Glucosinolates; Camalexin; Salicylic acid; Non-host resistance; SYSTEMIC ACQUIRED-RESISTANCE; BINDING CASSETTE TRANSPORTER; SALICYLIC-ACID; NONHOST RESISTANCE; SECONDARY METABOLITES; GLUCOSINOLATE BIOSYNTHESIS; BOTRYTIS-CINEREA; ABC TRANSPORTER; HYPERSENSITIVE RESPONSE; DISEASE RESISTANCE;

D O I：

10.1186/s12915-017-0360-z

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Plants are exposed to diverse pathogens and pests, yet most plants are resistant to most plant pathogens. Non-host resistance describes the ability of all members of a plant species to successfully prevent colonization by any given member of a pathogen species. White blister rust caused by Albugo species can overcome non-host resistance and enable secondary infection and reproduction of usually non-virulent pathogens, including the potato late blight pathogen Phytophthora infestans on Arabidopsis thaliana. However, the molecular basis of host defense suppression in this complex plant-microbe interaction is unclear. Here, we investigate specific defense mechanisms in Arabidopsis that are suppressed by Albugo infection. Results: Gene expression profiling revealed that two species of Albugo upregulate genes associated with tryptophanderived antimicrobial metabolites in Arabidopsis. Albugo laibachii-infected tissue has altered levels of these metabolites, with lower indol-3-yl methylglucosinolate and higher camalexin accumulation than uninfected tissue. We investigated the contribution of these Albugo-imposed phenotypes to suppression of non-host resistance to P. infestans. Absence of tryptophan-derived antimicrobial compounds enables P. infestans colonization of Arabidopsis, although to a lesser extent than Albugo-infected tissue. A. laibachii also suppresses a subset of genes regulated by salicylic acid; however, salicylic acid plays only a minor role in non-host resistance to P. infestans. Conclusions: Albugo sp. alter tryptophan-derived metabolites and suppress elements of the responses to salicylic acid in Arabidopsis. Albugo sp. imposed alterations in tryptophan-derived metabolites may play a role in Arabidopsis nonhost resistance to P. infestans. Understanding the basis of non-host resistance to pathogens such as P. infestans could assist in development of strategies to elevate food security.

引用

页数：22

共 109 条

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