Detection of Mycobacterium tuberculosis in paucibacillary sputum: performances of the Xpert MTB/RIF ultra compared to the Xpert MTB/RIF, and IS6110 PCR

被引：33
|
作者：
Kolia-Diafouka, P. [1 ]
Carrere-Kremer, S. [1 ]
Lounnas, M. [2 ]
Bourdin, A. [3 ]
Kremer, L. [4 ]
Van de Perre, P. [1 ]
Godreuil, S. [2 ]
Tuaillon, E. [1 ]
机构：
[1] Univ Montpellier, CHU Montpellier, INSERM, Pathogenesis & Control Chron Infect,EFS, Montpellier, France
[2] Univ Montpellier, CHU Montpellier, CNRS, UMR MIVEGEC IRD,Ctr IRD, Montpellier, France
[3] Univ Montpellier, CHU Montpellier, CNRS, PhyMedExp,INSERM,U1046,UMR 9214, Montpellier, France
[4] Univ Montpellier, CNRS, INSERM, IRIM,UMR9004, Montpellier, France
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2019年 / 94卷 / 04期
关键词：
Chelex (R); Mycobacterium tuberculosis; IS6110-PCR; Xpert MTB/RIF; Xpert MTB/RIF ultra; Paucibacillary sputum; REAL-TIME PCR; MEDIATED ISOTHERMAL AMPLIFICATION; DIAGNOSTIC-ACCURACY; ASSAY; MENINGITIS; EXTRACTION; COMPLEX; TESTS; DNA;
D O I：
10.1016/j.diagmicrobio.2019.02.008
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The Xpert MTB/RIF Ultra assay has recently been launched to improve the detection of smear negative disease. This retrospective study compares the sensitivity of Xpert MTB/RIF Ultra with that of Xpert MTB/RIF tests and IS6110 real-time PCR in sputum. Diagnostic performance of three molecular tests was evaluated using 48 culture-positive clinical respiratory specimens diluted to obtain paucibacillary sputum specimens. Xpert MTB/RIF Ultra had the highest sensitivity of 100% compared to 42% (P < 0.001) for Xpert MTB/RIF and 64.5% (P = 0.02) for IS6110-PCR. All "very low" or "low" positive specimens using Xpert MTB/RIF Ultra were tested positive using IS6110-PCR, but 35.4% were found negative using Xpert MTB/RIF. Xpert MTB/RIF Ultra is more sensitive than the two other tests for sputum with a low bacterial load. Adding detection of IS6110 and IS1081 to rpoB, is a key evolution of the assay and improves the detection of Mycobacterium tuberculosis' DNA in paucibacillary sputum. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码：365 / 370
页数：6
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