Ecological study of lactic acid microbiota isolated from Tempranillo wines of Castilla-La Mancha

被引：33

作者：

Izquierdo Canas, Pedro Miguel ^{[2
]}

Ruiz Perez, Patricia ^{[1
]}

Sesena Prieto, Susana ^{[1
]}

Palop Herreros, Maria Llanos ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Fac Ciencias Medio Ambiente, Dept Quim Analit & Tecnol Alimentos, Toledo 45071, Spain

[2] Inst Vid & Vino Castilla La Mancha, Ciudad Real 13700, Spain

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 108卷 / 03期

关键词：

Malolactic fermentation; Oenococcus oeni; Lactic acid bacteria; Wine; Genetic characterisation; RAPD-PCR; AMPLIFIED POLYMORPHIC DNA; OENOCOCCUS-OENI STRAINS; FIELD GEL-ELECTROPHORESIS; MALOLACTIC FERMENTATION; GENETIC DIVERSITY; RAPD-PCR; BACTERIA; IDENTIFICATION; SELECTION;

D O I：

10.1016/j.jbiosc.2009.04.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genetic diversity of 339 strains of lactic acid bacteria responsible for the spontaneous malolactic fermentation (MLF) in Tempranillo wines produced in six cellars located in different provinces of Castilla-La Mancha region of Spain was analyzed by using randomly amplified polymorphic DNA (RAPD). The results indicate that Oenococcus oeni was the predominant species in MLF of Tempranillo wines with a scant diversity of other lactic acid bacteria (LAB) species. The presence of a well-adapted cellar microbiota is confirmed, with MLF dominated by a limited number of O. oeni genotypes. Clustering of isolates from samples taken from different provinces showed the existence of coincident genotypes in different cellars of the region. This result suggests that a population of cosmopolitan O. oeni strains exists in the Castilla-La Mancha wine-growing region. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：220 / 224

页数：5

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