Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein

被引：8

作者：

Backofen, B

Jacob, R

Serth, K

Gossler, A

Naim, HY

Leeb, T

机构：

[1] Hannover Sch Vet Med, Inst Anim Breeding & Genet, D-30559 Hannover, Germany

[2] Hannover Sch Vet Med, Inst Physiol Chem, D-30559 Hannover, Germany

[3] Hannover Med Sch, Inst Mol Biol, D-3000 Hannover, Germany

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2002年 / 269卷 / 21期

关键词：

nicolin; comparative genomics; human; mouse; dog;

D O I：

10.1046/j.1432-1033.2002.03232.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a novel mammalian gene, termed nicolin 1 gene (NICN1), that is present in human, dog and mouse, whereas it is absent from the available genome sequences of nonmammalian organisms. The NICN1 gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein (GFP)-tagged nicolin 1 fusion proteins indicate that nicolin 1 is a nuclear protein. Northern analysis and semiquantitative RT-PCR demonstrated that the 2.5 kb NICN1 mRNA is expressed in a tissue-specific manner. The highest NICN1 expression levels are found in brain, testis, liver, and kidney. On the other hand the NICN1 expression is weak in spleen, leukocytes, small intestine and colon. The NICN1 gene is also expressed during development.

引用

页码：5240 / 5245

页数：6

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