Acetylation and level of mitochondrial transcription factor A in several organs of young and old rats

被引：55
作者：
Dinardo, MM
Musicco, C
Fracasso, F
Milella, F
Gadaleta, MN
Gadaleta, G
Cantatore, P
机构：
[1] Univ Bari, Dipartmento Biochim & Biol Mol, I-70125 Bari, Italy
[2] CNR, Ist Biomembrane & Bioenerget, I-70125 Bari, Italy
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 301卷 / 01期
关键词：
mtDNA; TFAM; aging; copy number; acetylation;
D O I：
10.1016/S0006-291X(02)03008-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single lysine residue and that the level of acetylation did not change with age. The measurement of the content of TFAM and of mitochondrial DNA (mtDNA) in several organs (cerebellum, heart, kidney, and liver) of young and old rats showed an age-related increase of mtDNA and TFAM in all the organs analyzed, except in heart. These data are discussed in the light of the multiple roles of TFAM in mitochondrial biogenesis and of the age-related change of the mitochondrial transcription. (C) 2002 Elsevier Science (USA). All rights reserved.
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收藏
页码：187 / 191
页数：5
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