CODON USAGE BIAS ANALYSIS FOR ABIOTIC STRESS GENES IN ARABIDOPSIS THALIANA AND ORYZA SATIVA

Codon usage bias (CUB) directly affects the expression level of protein. The protein expression also affects by abiotic stresses such as cold, drought, heat, salt, dehydration, heavy metals and oxidative stresses that reduce the productivity of plants. The current research is based to study the relationship of the CUB analysis for abiotic stress responding and housekeeping genes in Arabidopsis thaliana and Oryza sativa. A total of 159 genes (including 138 abiotic stress resistant and 21 housekeeping genes) were subjected for the CUB analysis. Combinations of various bioinformatics tools were applied for the CUB analysis of abiotic stress resistant and housekeeping genes. Out of 159, the 92 abiotic stress resistant and 16 housekeeping genes showed similarities by more than 50% for the usage of codons in Arabidopsis thaliana and Oryza sativa. The most preferred codon usage among these stress resistant and housekeeping genes were also calculated. The CUB analysis and most preferred codons findings will be helpful to engineer the abiotic stress resistant crops through adjusting specific codon usage that enhance yield and productivity of significant plants under biotic and abiotic stresses.