Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

被引：377
作者：
Reetz, Manfred T. [1 ]
D Carballeira, Jose [1 ]
Vogel, Andreas [1 ]
机构：
[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Ruhr, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 46期
关键词：
biotransformation; directed evolution; enzymes; protein engineering; protein stability;
D O I：
10.1002/anie.200602795
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Efficiency counts: A new method of directed evolution for increasing the thermostability of proteins saves time and effort. In an illustrative study, iterative cycles of saturation mutagenesis were performed at those sites in a lipase that display high B factors. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：7745 / 7751
页数：7
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