Hypoglycemic ingredients identification of Rheum tanguticum Maxim. ex Balf. by UHPLC-triple-TOF-MS/MS and interrelationships between ingredients content and glycosidase inhibitory activities

被引：11

作者：

Yue, Huilan ^{[1
]}

Jiang, Sirong ^{[1
,2
]}

Wang, Luya ^{[1
,2
]}

Banma, Cailang ^{[1
]}

Zhou, Guoying ^{[1
]}

Shao, Yun ^{[1
]}

Tao, Yanduo ^{[1
]}

Zhao, Xiaohui ^{[1
,2
]}

机构：

[1] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2022年 / 178卷

基金：

中国国家自然科学基金;

关键词：

Rheum tanguticum Maxim. ex Balf; UHPLC-Triple-TOF-MS/MS; Hypoglycemic ingredients identification; Interrelationships; Glycosidase inhibitory activities; ALPHA-GLUCOSIDASE INHIBITORS; POSTPRANDIAL HYPERGLYCEMIA; CONSTITUENTS; ACARBOSE;

D O I：

10.1016/j.indcrop.2022.114595

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Rheum tanguticum Maxim. ex Balf. (RT), mainly is mainly produced in Gansu, Qinghai, Tibet and Sichuan of China, has long been used as a medicine for purging fire and quenching thirst. The RTR-2 (Rheum tanguticum root extract without polysaccharides and protein) had strong inhibitory activities against a-amylase, maltase and sucrase with the IC50 0.042 mg/mL, 0.124 mg/mL and 0.097 mg/mL, respectively. In addition, RTR-2 could significantly reduce PBG level of diabetic mice in starch/maltose/sucrose tolerance test. Total 48 constituents including 12 anthraquinones, 4 bianthmnes, 8 flavans, 3 stilbenes, 5 phenylbutanones, 4 naphthosides, 8 glucose gallates and 4 phenolic acids were determined in RTR-2 by UPLC-Triple-TOF/MS analysis of molecular ion, main fragments, retention time, and references. Furthermore, the components such as flavan ((-)-epicatechin 3-O-gallate) and phenylbutanone (lindleyin) were demonstrated to have sucrase and maltase inhibitory activites, and might be the main hypoglycemic active ingredients of RTR-2 extract. All these results offered an exhaustive knowledge for the hypoglycemic compounds of Rheum tanguticum, which was conducive to its further development and utilization as a valuable source of hypoglycemic plant in pharmaceutical industry.

引用

页数：12