Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv., and a survey of variation from Europe and Asia

被引:30
作者
Fukunaga, K
Domon, E
Kawase, M
机构
[1] SHIKOKU NATL AGR EXPT STN,DEPT CROP IMPROVEMENT,LAB BARLEY BREEDING,KAGAWA 765,JAPAN
[2] SHIKOKU NATL AGR EXPT STN,DEPT CROP IMPROVEMENT,LAB PLANT BIOTECHNOL,KAGAWA 765,JAPAN
关键词
foxtail millet; rDNA; RFLP; restriction mapping; geographical distribution;
D O I
10.1007/s001220050621
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Restriction fragment length polymorphism (RFLP) and the structure of ribosomal RNA genes (rDNA) were investigated in 117 landraces of foxtail millet, Setaria italica (L.) P. Beauv. Five RFLP phenotypes were found when the genomic DNA was digested with BamHI; these were named types I-V. Of these types I, II and III were the most frequent. Type I was mainly distributed in the temperature zone, type II in the Taiwan-Philippines Islands and type III in South Asia. Restriction mapping of the cloned rDNA and comparison with RFLP phenotypes showed that the different types originated from a polymorphism in the length within the intergenic spacer (IGS) and BamHI site changes within the IGS.
引用
收藏
页码:751 / 756
页数:6
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